Research On The Effects Of Taohongsiwu Decoction On Rabbit Skin Tissue Transplantation Model Of VEGFR-2Levels

Objective:By observing the taohongsiwu decoction on rabbit skin graft, the transplant flap of changes in general, histopathological situation and the change of vascular density detection and VEGFR-2content, and analyze the correlation of VEGFR-2levels and vascular density,explore taohongsiwu decoction’s influence on transplant flap survival, and angiogenesis mechanism.Methods:Healthy New Zealand white rabbit(CL)60, according to random number table method is divided into two groups:treatment group and control group. Made mold, skin grafting were performed in both groups, treatment group after building to taohongsiwu decoction lavage for2weeks, control group to fill the stomach of the distilled water for2weeks. Then respectively to Building after3d,5d,14d randomly selected10from each group, observing the transplant flap of changes in general and specimens for histopathological examination and the change of vascular density detection, using immunohistochemical method to detect the contents of skin of VEGFR-2.Results:Postoperative3d,5d,14d, the transplant flap of changes in general and pathological observation under light microscopy, the treatment group were superior to control group; Transplant skin histopathological grading and vascular density test, each time in treatment group were higher than control group, the difference was statistically significant (P＜0.01); Transplant skin of VEGFR-2content detection, early postoperative rise gradually, then gradually decreased, the treatment group compared with control group the difference was statistically significant (P＜0.05); Transplant skin VEGFR-2levels and vascular density of correlation analysis, early postoperative was positively linear correlation (P＜0.05).Conclusion:After Rabbit skin graft, taohongsiwu decoction can significantly improve and heighten histopathological grading, increase the density of blood vessels, improve VEGFR-2levels, to promote their transplantation flap survival, angiogenesis and its mechanism may be through improved early postoperative skin of VEGFR-2levels, increase the density of blood vessels, thus promote the transplant flap survival.