The Expression And Clinical Significance Of AQP1and Nm23-H1in Endometrial Adenocarcinoma

Objective: The goal of this article is to research AQP1andnm23-H1protein detected by immunohistochemistry in normalendometrium, simple and complex hyperplasia, atypical endometrialhyperplasia and endometrial adenocarcinoma and research the expressionlevel in different tissues above situation with histopathological grading,surgical-pathologic stage, myometrial relationship between the depth ofinvasion, and lymph node metastasis by statistical test method. Exploreits role in the occurrence, development and prognosis of endometrialadenocarcinoma, and to provide a reference for the early diagnosis ofendometrial adenocarcinoma, treatment and condition assessment andprognostic evaluation.Methods: Selected the tissue specimens by surgical resection in The China-Japan Union Hopsital of Jilin University between September2010and September2012. Select the specimen were freshly drawnimmediately fixed in10%neutral formalin solution, paraffin embedded.Slice4μm thick, stained by HE,AQP1and nm23-H1immunohistochemical staining. Positive control for known positivesections contained in the kit, pathologists identified all slices by blinded,immunohistochemistry staining tissue to detection AQP1and nm23-H1expression.Results:1. AQP1protein positive particles brownish yellow, locatedin the cell membrane and (or) nucleus. The positive expression of AQP1in Group1, Group2, Group3, Group4, Group5is6.7%,10.0%,13.3%,16.7%,37.9%. Compared among the five groups,χ2=17.751, P<0.01，there was a significant statistical difference. AQP1protein expression was significantly elevated in endometrialadenocarcinoma, there was a statistically significant difference comparedwith the remaining four groups, the results were χ2=9.744, χ2=7.584,χ2=5.747, χ2=4.204, P＜0.05. Compared among1-4Group, χ2=1.617, P ＞0.05, there was no significant difference or statistically significant,.2. nm23-H1protein positive particles brownish yellow, located inthe nucleus. The positive expression of nm23-H1in Group1, Group2,Group3, Group4, Group5is73.3%,66.7%,56.7%,50.0%,34.5%.Compared among the five groups, χ2=15.876, P<0.01， there was asignificant statistical difference. AQP1protein expression wassignificantly lower in endometrial adenocarcinoma, there was astatistically significant difference compared with the remaining fourgroups, the results were χ2=11.962, χ2=8.261, χ2=5.274, χ2=3.993, P＜0.05. Compared among1-4Group, χ2=2.138, P＞0.05, there was nosignificant difference or statistically significant.3. The positive expression rate of AQP1protein in surgical stage Iand II~III were27.5%,61.1%. Compared between the two group,χ2=5.957, P＜0.05, there was a significant statistical difference. Thepositive expression rate of AQP1protein in histopathological grading G1and G2+G3were23.3%,53.6%. Compared between the two group,χ2=5.625, P＜0.05, there was a significant statistical difference. The positive expression rate of AQP1protein in≤1/2depth of myometrialinvasion and＞1/2depth of myometrial invasion were25.0%,59.1%.Compared between the two group, χ2=6.741, P＜0.05, there was asignificant statistical difference. The positive expression rate of AQP1protein in without lymph node metastasis and lymph node metastasiswere26.7%,66.7%. Compared between the two group, χ2=5.815, P＜0.05, there was a significant statistical difference.4. The positive expression rate of nm23-H1protein in surgical stageI and II~III were45.9%,11.1%. Compared between the two group,χ2=6.310, P＜0.05, there was a significant statistical difference. Thepositive expression rate of nm23-H1protein in histopathological gradingG1and G2+G3were50.0%,17.9%. Compared between the two group,χ2=6.623, P＜0.05, there was a significant statistical difference. Thepositive expression rate of nm23-H1protein in≤1/2depth of myometrialinvasion and＞1/2depth of myometrial invasion were44.4%,18.2%.Compared between the two group, χ2=4.169, P＜0.05, there was asignificant statistical difference. The positive expression rate of nm23-H1 protein in without lymph node metastasis and lymph node metastasiswere43.2%,8.3%. Compared between the two group, χ2=4.725, P＜0.05,there was a significant statistical difference.Conclusion:1. AQP1expression may promote the development ofendometrial adenocarcinoma.2. nm23-H1low expression may promote the development ofendometrial adenocarcinoma.3. High expression of AQP1may indicate a high degree ofmalignancy and invasion intensity.4. Low expression of nm23-H1may indicate a high degree ofmalignancy and invasion intensity.