Sequence Analysis Of EGFR, MET, KRAS And BRAF Genes’ Hotspot Mutation In Gastric Cancer

Objective:EGFR、MET、KRAS and BRAF are frequently activated in the development of multiple kinds of malignant tumors. A multitude of inhibitors and clinical researches are been proceeding at present, especially in western countries. However, the incidences of the hotspot mutations in these genes in China are still unclear. In the present study, we detected the hotspot mutations of EGFR, KRAS, BRAF and MET in Chinese patients with gastric cancer.Methods:(1)41cases of gastric adenocarcinoma were collected. The41patients accepted D2operations in Fudan university affilliated Zhongshan hospital between April2011and July2011, and all of them did not undergone anticancer therapy before operation.(2) DNA was extracted from the tumor tissues. PCR was used to amplify the exons of the genes, in which the hotspots existed.(3) Sanger sequencing was used to detect the hotspot mutations of these four genes.Results:(1) Among the41patients, two of them, number1143and1156, have non-sense mutation Q787Q in EGFR (4.9%,2/41). Besides Q787Q mutation, G12D non-synonymous mutation was also detected in sample1143. No mutation was detected in both MET and BRAF genes.Conclusion:In Chinese patients with gastric carcinoma, the incidence of hotspot mutations in EGFR、MET、KRAS and BRAF genes is very low. Consequently, these hotspot mutations play very limited roles in the development of gastric cancer in China. Taken together, new effective molecular targets should be needed in the treatment of gastric carcinoma.