The Effects Of CaM Binding Peptide Of Sema4D On The Proliferation, Adhesion And Migration Of Hela Cells As Well As On The Angiogenesis Of HUVEC

Calmodulin (CaM) plays an important role in the proliferation, adhesion and migration of tumor. It is reported recently that CaM is also related to tumor angiogenesis. Cervical carcinoma is one of the most common malignant tumors with the second highest morbidity ranked only lower to breast cancer in women. However, reports about the role and the mechanism of CaM in cervical carcinoma are relatively rare. We recently identified a region within the cytoplasmic domain of semaphorin4D that binds calmodulin in platelets. We presume that CaM binding peptide of Sema4D (CBPS) corresponding to this region may have effect on tumor and angiogenesis.To explores the effects of CaM binding peptide of Sema4D (CBPS) on the proliferation, adhesion and migration of HeLa as well as on the angiogenesis of HUVEC cells. Besides, we also want to explore the molecular mechanism of the effects of CBPS. HeLa and HUVEC were cultured in RPMI-1640medium.1. The effects of CBPS on the proliferation, adhesion and migration of HeLa cells were measured with the methods of MTT, cell adhesion assay, wound healing and cell migration assay, respectively.2. The effects of CBPS on the angiogenesis of HUVEC were detected with tube formation assay.3. Phosphorylation of ERK in HeLa and HUVEC was detected with Western-blot.We found that compared with control groups, the proliferation, adhesion and migration of HeLa cells was decreased significantly after CBPS treatment. Besides. CBPS significantly inhibited the angiogenesis of HUVEC cells. CBPS significantly inhibited the phosphorylation of ERK in HeLa and HUVEC cells after CBPS treatment.In summary, CBPS can inhibit the proliferation, adhesion and migration of HeLa cells as well as inhibit the angiogenesis of HUVEC cells. CBPS inhibit the proliferation, adhesion and migration of HeLa cells and the angiogenesis of HUVEC cells through inhibiting the phosphorylation of ERK.