Effects Of Lead Acetate On Apoptosis Of Human Liver Cell Line L-02and Expression Of Caspasc-3

Objective: To study the the relationship between lead acetate on apoptosis of cultured human liver cell line L-02cells and expression of caspase-3.Methods: After treated L-02cells at24h\48h with different concentrations (0、2.5、40、100、200、400umol/L), inhibitory effect of lead acetate on the proliferation of L-02cells was assayed by MTT method. The morphologic changes after lead acetate treatment were observed by Hochcst33258staining; the expression of caspase-3mRNA was estmiatcd by RT-PCR and Western blot.Results: Compared to normal control group after24h\48h, The proliferation of L-02cells was significantly inhibited treated with lead acetate (24h0.4678±0.0438,0.4686±0.0733,0.4500±0.0712,0.4244±0.0407,0.399810.0499vs0.5234±0.0589;48h0.4290±0.0607,0.4184±0.0296,0.4032±0.0499,0.3856±0.0386,0.3750±0.0149vs0.5300±0.0397, P<0.05or0.01); Hochest33258staining showed morphological changes in lead acetate group after48h. The levels of caspasc-3mRNA transcription were significantly higher than the normal control group, and its role showing a clear dose-effect relationship (1.0912±0.0769,1.2874±0.144,1.4536±0.1046,1.6986±0.1371,1.9882±0.0925vs0.8438±0.0933, P<0.01). Compared to normal control group. the expression levels of caspase-3protein were increased.Conclusions:Lead acetate can inhibit the proliferation and induce apoptosis of L-02cells, and the mechanism may be related with the activation of caspase-3.