The Experimental Research Of Nicotine On The Impact Of The Surviving Time Of Rats With SIRS

Objective:To explore the effects of nicotine on surviving time of rats with systemic inflammatory response syndrome (SIRS) induced by hemorrhagic shock and LPS.Methods:100male SD rats were randomly divided into five groups (each group, n=20).7ds before rat models were induced by SIRS:the control group doen’t receive any treatment,the vagus transection group of right neck cut off from vagus trunk of the right neck, pretreatment group with nicotine given daily by intraperitoneal nicotine solution (5mg/kg) injection rats with both vagus trunk of right neck cut off and pretreatment by nicotine given daily nicotine solution (5mg/kg) by intraperitoneal injection; The nicotine treatment group:they were given nicotine solution (5mg/kg) by intraperitoneal injection everyday after induction of SIRS.The incision of the right neck vagus trunk was adopted by cutting along the midline of the neck to exposing. Production of " two-hits "(hemorrhagic shock and intraperitoneal injection of LPS) rat models with SIRS.To collect blood specimens or organs for associated determination, and compare HMGB-1levels of those five groups of rats’serum,their surviving time and the pathological changes of the process of Colon going back to cecum.Results:Rats with nicotine treatment survive the least time due to least concentration of HMGB-1. Rats with nicotine pretreatment survive the longest time due to low concentration of HMGB-1and the least harm to colon.; Rats with the vagus trunk of right neck cut off survive short time due to high concentration of HMGB-1and the worst harm to colon.The concentration of HMGB-1degree of colon damage and rats’surviving time in the group with combined treatment was between group B and C. And there were obviously statistical significance comparing with the control group.Conclusion:Nicotine may alleviate the inflammatory reaction of rats with SIRS, protect important organs and postpone the survival time of rats. The surviving time of SIRS rats may be a negative correlation to the HMGB-1concentration of late inflammatory mediator...