The Circulating Levels Of RAVTES, IL-17,α-synuclein And CD4~+CD45RO~+T Cells In Parkinson’s Disease

ObjectiveTo detect the circulating concentrations of RANTES,IL-17, α-syn and thepercentage of CD4~+CD45RO~+T cells in Parkinson’s disease, and to investigatethe causes of those changes in PD as well as the influencing factors of them.MethodsThe serum RANTES, IL-17, α-syn levels of43PD patients and33healthysubjects with age and sex-matched were measured by Enzyme-LinkedImmunolsorbent Assay(ELISA). And the percentage of circulating CD4~+CD45RO~+T cells were detected by using two-colour flow cytometry. The UnitedParkinson’s Disease Rate Scale Part Ⅲ Score（UPDRS Ⅲ）were evaluated in allstudied PD patients. And all PD patients were divided into several subgroupsaccording to the different gender,age,duration and treatment status.ResultsThe mean serum RANTES,IL-17, α-syn levels and the percentage of CD4~+CD45RO~+T cells of PD group are significantly higher than the healthy subjectsgroup (all P<0.001), moreover, a strong and significant correlation betweenRANTES and UPDRS Ⅲ score was observed in patients with PD(Rs=0.74, P <0.00001),as well as in IL-17and UPDRS Ⅲ score (Rs=0.52, P <0.01). The PDgroup were also divided into several different subgroups according to differentthe gender,age,duration and treatment status,however,there is no significant differences in all subgroups respectively（all P>0.05）. There were positivecorrelations found between RANTES and the percentage of CD4~+CD45RO~+Tcells observed in PD group (Rs=0.53, P <0.01),while negative correlationswere observed between α-syn and IL-17(Rs=0.34, P <0.05).ConclusionsThe PD group presented with significantly higher RANTES,IL-17,α-syn andthe percentage of CD4~+CD45RO~+T cells levels. And both RANTES and IL-17levels were strongly and significantly correlated with the PD clinical subjectiveevaluation.However, the gender, the age, the duration and the treatment statuscannot interfere the circulating levels of any of them. In PD group, There werepositive correlations between RANTES levels and the percentage of CD4~+CD45RO~+T cells, while negative correlations between α-syn and IL-17levels.Our findings may suggest that all those immunity factors were detected in ourresearch may implicate in PD. RANTES, secreted by activated microglia, maycontribute to increasing the number of circulating CD4~+T cells and may alsoinducing CD4~+T cells infiltrating at the sites of neuronal injury. While α-synuclein,released by dead nigral dopaminergic neurons, may through inducing Th17adaptive immune response，which as a new member of T helper cells,maycontribute to PD progressing. It also implied that activated microglia and theadaptive immunity responses induced by CD4~+T lymphocytes may play animportant role in the pathogenetic mechanisms of PD.