Experimental Study Of Extract From Fuctus Mume To Prevent Calcium Oxalate Stones In Male Rats

ObjectiveResearch the preventive effect of Extract from Fuctus Mume on calciumoxalate stone formation in male rats and to discuss the mechanism.MethodsForty Health male SD rats were randomly divided into five groups(N=8each),they were control, model, and Fuctus Mume intervention groups.Afterexperiment2weeks，the24h urine concentration Ox、Ca2+,the concentration ofserum BUN、Cr、Ca2+、P,and MDA、SOD of renal tissue were detected in eachof group rats,The calcium oxalate deposit in the kidney was observed bymicroscopy.Immunohistochemistry were used to assess the protein expressionof OPN in rat renal tissue of every groups.Results1.Urine test index results:24h urine concentration Ox,Ca2+of stoneformation group and Extract from Fuctus Mume intervention group wereobviously higher than control group (P<0.01); Extract from Fuctus Mumeintervention group was lower than stone formation group obviously(P<0.01).2.Bloodtest index results: Serum concentration of BUN, Cr show that groupB、C、D and E were obviously higher than control group (P<0.01).Comparedwith group B, the group C、D and E serum concentration of BUN weredecreased, but it is not statistically significant differences (P>0.05)and serumconcentration of Cr were significantly decreased (P<0.01).Compared with group C, the group D、E serum concentration of BUN, Cr were decreased, but it is notstatistically significant differences (P>0.05).The serum concentration of Ca2+andP showed no significant difference in all rats（P>0.05).3.MDA and SOD activity:The enzyme activity of superoxide dismutase(SOD) of renal tissue in stone formation and Fuctus Mume intervention groupwere lower than control group(P<0.01).The enzyme activity of superoxidedismutase (SOD) of renal tissue in Fuctus Mume intervention group was higherthan stone formation group(P<0.05).Compared with group C,the group D、Eenzyme activity of superoxide dismutase(SOD)were rose,but it is not statisticallysignificant differences(P>0.05).The concentration of malondialdehyde(MDA) ofrenal tissue in stone formation and Fuctus Mume intervention group were higherthan control group(P<0.01).The concentration of malondialdehyde (MDA) ofrenal tissue in stone formation group was higher than Fuctus Mume interventiongroup(P<0.05).Compared with group C,the groupD、 E concentration ofmalondialdehyde (MDA) of renal tissue were decreased, but it is not statisticallysignificant differences (P>0.05).4.HE and Von Kossa,s dyeing and observe the pathological change ofkideny:Von Kossa,s method clearly demonstrated the presence of pitchycalcium oxalate deposits in the kidney of the stone formation group and FuctusMume intervention group.No deposits were detected in the control group.Thetubular dilatation in the kidney was fended on stone formation group and FuctusMume intervention group.Quantitative analysis of the density of calciumdeposits showed a significantly lower density of deposits in the Fuctus Mumeintervention group compared to the stone formation group.5.OPN protein immunohistochemical results: In rats of normal control group,only a weak OPN protein staining was noted.The expression was enhanced inthe stone group.Extract from Fuctus Mume-treated rats tended to have lowerOPN protein expression than the stone group. Conclusion1.The extracts from Fuctus Mume can block the formation of renal calciumoxalate stone in male rats potently.2.The mechanism may be to protect renal tubular epithelial cells fromdamage by free radical by hyperoxaluria.and to reduce the formation of calciumoxalate stone in male rats.3.The extracts from Fuctus Mume may low down OPN to reduce theformation of calcium oxalate stone in male rats.