Effect Of FGF10Monoclonal Antibodies Against The Psoriasis In Human Skin/SCID Mouse Xenograft Model

Psoriasis is a T cell-mediated chronic inflammatory dermatosis, T lymphocyte infiltration in the dermis is not only the causative link of the pathogenesis, but also the important factor to maintain the psoriatic lesions characteristic. In addition to T cells, dendritic cells, monocytes/macrophages, and some chemokines and cytokines are involved in immune processes of psoriasis, resulting in keratinocyte proliferation and dermal infiltration of immune cells and other pathological changes.Keratinocytes (KC) abnormal proliferation of psoriatic lesions, which is the most characteristic performance, is regulated by many factors. Effectively inhibition of KC proliferation will lead to a improvement effect of psoriasis. The cytokines in pathogenesis of psoriasis, such as IFN-y, IL-6, IL-8, IL-22promote epidermal hyperplasia, at the same time, epidermal growth factor (EGF), transforming growth factor a (TGF-a), and fibroblast growth factors (FGFs) are important regulators of keratinocyte proliferation. FGF10is highly expressed in psoriatic lesionsObjective:Exploring the FGF10monoclonal antibody partial packets therapeutic effect of the psoriasis in SCID xenogeneic transplantation mouse model, investigate the effects of FGF10monoclonal antibody treatment of psoriasis, in order to provide a theoretical basis of the possible mechanisms.Methods:Transplanting vulgaris or plaque psoriasis lesions in SCID mice to make the psoriasis in SCID xenogeneic transplantation mouse model. Set the positive control group (the hydrocortisone butyrate treatment group), the treatment group (FGF10antibody (0.188mg/ml) group), and the negative control group (vaseline base group). SCID mice were sacrificed after3days of packet treatment, using the microscopy to evaluate the therapeutic effect, from three aspects of the skin lesions Baker score, the acantholysis cells numbers and dermal perivascular inflammatory cells count。Results:Compareing skin lesions Baker score and acantholysis cells numbers of three groups, there were no significant difference (P>0.05), no statistically significance; Compareing inflammatory cells count of the treatment group to the negative control group, there was a significant difference (P=0.020), Compareing inflammatory cells count of the positive control group to the negative control group, there was a significant difference (P=0.003),too. But the treatment group and the positive control group of inflammatory cells count was no significant difference (P>0.05).Conclusions:FGF10monoclonal antibody partial packet therapeutics could inhibit the dermal perivascular inflammatory cell infiltration in the pathological changes of psoriasis in SCID xenogeneic transplantation mouse model.