Study On The Effect Of The Expression Of Aquaporins And Intestinal Neurotransmitter By Biansetong Mixture In Constipated Rat’s Colon

Object: To establish the model of slow transit constipation(STC) anddetect the expression of AQP1, AQP8, AQP9,5-HT, NOS1in constipated rats,proximal colon.To research the mechanism of Biansetong Mixture in thetreatment of STC.Methods:60SD rats were randomly divided into5groups: blank group,model group, mosapride group, Maren pill group, Biansetong Mixture group.Blank group was given normal saline by gastric irrigation,while model groupand treatment groups were given Diphenoxylate by intragastric administrationbefore treated. When established the model of STC, the treatment groupswere respectively given mosapride, Maren Pills, Biansetong Mixture, Toobserve the stool grain and stool wet weight. Finally killed the rats collectiveand collected the rats,proximal colons. To detect expression of AQP1, AQP8,AQP9,5-HT, NOS1in the proximal colon by immunohistochemistry. Thestaining results were analyzed semi-quantitively by computerized color imageanalyzer using the following parameter:mean density.Results:(1) After the last administration to establish the model of STC,Both of stool wet weight and grain number of the blank control group werehigher than the other four groups，(p <0.05), the difference was statisticallysignificant, which show that we had established the rat model of slow transitconstipation successfully.(2) HE staining showed: the colonic mucosa ofblank group rats were normal, while the colonic mucosa of model group rats had the infiltration of inflammatory cells. Compared with blank controlgroup,the nerve cells in colonic myenteric plexus of model groupdecreased.(3) Immunohistochemical results: All of The density means(MD)of AQP1、AQP8、5-HT、NOS1in the colon of model group were greater thanthe blank control group, mosapride group, Maren pill group, BiansetongMixture group (p <0.05), the difference was statistically significant; Thedensity means(MD) of mosapride group, Maren Pill group, BiansetongMixture group were higher than blank control group (p <0.05), the differencewas statistically significant. The density means(MD) of AQP9in the colon ofmodel group was lower than the blank control group, mosapride group, Marenpill group, Biansetong Mixture group (p <0.05), the difference wasstatistically significant. The density means(MD) of mosapride group, MarenPill group, Biansetong Mixture group were lower than blank control group (p<0.05), the difference was statistically significant.Conclusions:(1) The increased expression of AQP1, AQP8anddecreased expression of AQP9in constipated rat,s colon may lead to reabsorbmore water and secrete less intestinal fluid. That caused the stool become dry.(2) The increased expression of5-HT, NOS1in constipated rats,colon may beinvolved in the pathogenesis of constipation.(3) The Biansetong Mixturecould make the expression of AQP1, AQP8,5-HT, NOS1decrease andexpression of AQP9increase in constipated rats,colon, which showed that thelaxative mechanism of Biansetong Mixture might be related to regulate theexpression of intestinal aquaporins and neurotransmitters.