The Impaction Of Sampling Tools On The Result Of Pathogen Detection

Objective: Compare the influence of three different sampling swab to escherichiacoli test results.Methord: E. coli cultures will be diluted to1:20,1:40,1:80,1:120four dilutiondegree, divided into cotton swab sampling route, sponge swab group, nylon flockinggroup and positive control group.Use ELISA method to detect residual protein in e.coli host, compare the suspension release ability and the release of homogeneitybetween groups of e. coli bacteria. At the same time, use the cotton swab, spongeswab and nylon flocking swab sampling four dilution degrees of e. coli cultures, theninoculated on blood plate, positive control group directly take the same amount of e.coli cultures inoculated on blood plate,5%CO2,37constant temperature culturewithin18h,compared the efficiency of different groups of bacteria culture.Result: The release ability of swab as absorbance value (OD value), in four dilutiondegrees,nylon flocking swab group of OD value compared with positive controlgroup, there was no statistically significant difference, compared with cotton swabgroup, there were statistical significant difference, in1:20dilution degrees, nylonflocking swab group of OD value compared with sponge swab group, there was nostatistically significant difference, in the other three dilution degrees compared withsponge swab group, there were statistical significant difference;In four dilutiondegrees,cotton swab group of OD value compared with positive control group, nylonflocking swab group and sponge swab group,there were statistical significantdifference, at1:120dilution degrees compared with negative control group, there was no statistical significant difference;in1:20dilution degree,sponge swab group of ODvalue compared wiht positive control group and nylon flocking swab group, therewere no statistical significant differences, in other dilution degrees, nylon flockingswab group compared with positive control group, there was statistical significantdifference. The release uniformity of swab is OD value of the coefficient of variation(CV),nylon flocking CV value is lower than the cotton swab,sponge swab CV valueslie somewhere in between. Swab sampling of Bacterial culture efficiency is expressedas a number of bacteria,In1:120dilution degrees nylon flocking swab group on thenumber of bacteria compared with positive control group,there was statisticalsignificant difference, in other dilution degrees compared with positive control group,there were no statistical significant differences,in the four dilution degrees comparedwith cotton swab group, there wase statistical significant difference, n1:20dilutiondegrees compared with sponge swab group, there was no statistical significantdifference, in other dilution degrees compared with cotton swab group, there wasstatistical significant difference;In four dilution degrees cotton swab group on thenumber of bacteria compared wiht positive control group, nylon flocking swab groupand sponge swab group,there were statistical significant differences;In1:20dilutiondegree,sponge swab group on the number of bacteria compared wiht positive controlgroup and nylon flocking swab group, there were no statistical significant difference,in other dilution degrees,positive control group compared with nylon flocking swabgroup,there was statistical significant difference.Conclusion: The absorption and release of e. coli suspension ability of nylon flockingswab is stronger than cotton swab and sponge swab, Sponge swab is between nylonflocking swabs and cotton swab,nylon,flocking swab is the good choice of clinicalspecimens to sent bacteria culture. Objective: Compare the result affection of syringe sampling method and cotton swabsampling tube sampling method of bacteroides fragilis culture.Method:1:20and1:40,1:80,1:120four dilution degrees of bacteroides fragilisbacteria suspension, sampling tool is divided into syringe, cotton swab group andpositive control group, take the same amount of bacteroides fragilis bacteriasuspension, vaccination, elution, use the real-time fluorescent quantitative PCR, andcompare the three groups of bacteria culture bacteria quantity (Ct value).Result: At each dilution degrees syringe group were visible colony growth, and thereis amplification curve appeared. Cotton swab group is not seen colony growth at1:120dilution degrees, no amplification curve, colony growth appear in both dilutiondegrees of each other, and have the amplification curve appeared. In1:20,1:40,1:80dilution degrees, syringe set of Ct value (20.35±0.08、24.78±0.65、27.30±0.15) areless than cotton swab sampling group of Ct value (24.20±0.46、31.80±0.35、37.78±0.31), the difference was statistically significant, and the Ct value of positivecontrol group (20.12±0.04、24.07±0.11、26.74±0.18), there were no statisticalsignificance difference; Cotton swab sampling group of Ct value is greater than the Ctvalue of positive control group, difference has statistical significance.Conclution: Syringe sampling anaerobic bacteria cultured bacteria amount is higherthan cotton swab sampling method.