Expression And Significance Of Trefoil Factor1in Kidney Tissue Of Rat With Calcium Oxalate Stone

ObjectiveTo Explore the expression of trefoil factor (TFF1) in kidney tissue of rat with calcium oxalate stone and to investigate the association with the mechanism of renal calculi formation.MethodRats were raised continually through drinking1%ethylene glycol solution and gavaging2%NH4CI solution for2weeks and4weeks; kidney stone formation was observed using Polarizing microscope;and expression of TFF1in renal tissue was detected by immunohistochemistry in two weeks group (n=20), four weeks group (n=20) and normal group (n=20) respectively.ResultsTFFlimmunoreactive substances mainly located in the glomerular and tubular compared with normal group, the TFF1average gray value decreased slightly (P>0.05) in two weeks group and significantly reduced in four weeks group(P<0.05).ConclusionExpression of TFF1was reduced in kidney tissue of rat with calcium oxalate stone. Accompanied by the increase in the number and density of stone crystalline,TFF1expression decrease shows that TFF1is a inhibitor of calcium oxalate stone formation. High level oxalic acid and COM crystals can cause TFF1expression profile changes in rat kidney.The changes of TFF1expression may protect epithelial cell from being injured, and may be related to kidney stone formation...