| Background and ObjectiveEssential hypertension (EH) is a common syndrome,its etiology includes both genetic and environmental factors. Generally believed that genetic factors accounted for40%and that environmental factors accounted for60%. Hypertension is an important cause of a variety of cardiovascular and cerebrovascular disease. Hypertension seriously affect vital organs’s structure and function, such as heart, brain and kidney. Long-term increased pressure load can stimulate myocardial cells hypertrophy and interstitial fibrosis. Hypertension is the main cause of left ventricular hypertrophy and expansion. In recent years, the incidence of hypertension is rising, hypertensive heart disease incidence is increasing too.Klotho gene is a new gene related to human aging closely, it was found by Kuro-o in1997. Due to the hnRNA shear difference, the protein that klotho gene express in humans and mice were divided into membrane type and secreted. The protein expressed mainly in the human body is secreted protein, the protein is considered to have hormone-like effects. The gene has relationship with a number of diseases which occur with aging. Studies have shown that Klotho gene polymorphism is associated with blood pressure regulation. This study aims at investigating the relationship between the Klotho gene G-395A polymorphism, C370S polymorphism, serum Klotho protein level, and serum NO level in hypertension patients with left ventricular hypertrophy(LVH).MethodsThis study selected76Essential Hypertension patients,82EH with LVH patients and69contral. Allele-specific primer(ASP)PCR technique was used to detect genotype of the klotho gene G395A and C370S SNP.Enzyme-linked immunosorbent assay was used to measure serum Klotho protein level, and chemical colorimetric method was used tomeasure serum NO level.ResultsThere was a significant difference in the three groups in the distribution frequency of Klotho gene G395A polymorphism(X2=16.976,P<0.05). The frequencies of A allele in the EH-LVH group and the EH group were significant different from the control group (P<0.05). And the distribution frequency of AA genetype in the EH-LVH group were higher than the control group(χ2=14.307, P<0.05).There was a significant difference in the three groups in the distribution frequency of Klotho gene C370S polymorphism(X2=15.827,P<0.05). The distribution frequency of SS genetype in the EH-LVH group were higher than the control group(x2=14.985, P<0.05). The frequencies of C allele in the EH-LVH group and the EH group were lower than the control group. The difference was statistically significant (P<0.05).The Klotho protein concentration in the EH patients with or without LVH was significantly lower than that of control (P<0.05).The NO concentration in the EH patients with or without LVH was significantly lower than that of control (P<0.05).In the three groups, the concentration of Klotho protein in GG genotype were higher than those in GA genotype and AA genotype (P<0.05),the concentration of Klotho protein in CC genotype were higher than those in CS genotype and SS genotype (P<0.05).ConclusionThe Klotho gene G395A polymorphism is associated with EH and EH-LVH patients. The AA genetype is liable to hypertension.The A allele may be a susceptibility gene for hypertension. The GG genetype may be an protective factor for hypertension and LVH. The Klotho gene C370S polymorphism is associated with EH and EH-LVH patients. The AA genetype is liable to hypertension.The A allele may be a susceptibility gene for hypertension. The GG genetype may be an protective factor for hypertension and LVH. The increase of plasma Klotho protein level might be a protective factor of hypertension and left ventricular hypertrophy caused by hypertension... |
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