The Effect Of Alkaloids From Rhizoma Coptis On Uncoupling Protein2

The diabetes and diabetic complications have become a seriously worldwide public health problem. Some researches indicate that the initiation and progression of diabetes and its complications are closely associated with oxidative stress. As a "power" of cell activity, mitochondria play an important role in energy metabolism, besides, it is also closely to the metabolism of reactive oxygen species (ROS) intracellular. As a widely distributed proton transport protein in the inner mitochondrial membrane, Uncoupling protein2(uncoupling protein2, UCP2) which has high proton translocation activity enables the protons directly into the mitochondrial matrix without produce ATP. The process consumes free energy store in the proton electrochemical potential energy, and avoids the production of reactive oxygen species.As a traditional Chinese medicine, Rhizoma coptidis has a long history and wide range of pharmacological. In the study, hypoglycemic activity, correlations between hydrastine and UCP2, antioxidant activity of four major alkaloids in Rhizoma coptidis were studied. The study will provide a theoretical basis for the further utilization of CoptisRhizoma.The results are listed as follow:1The affect of four alkaloids on expression of UCP2and antioxidant activityIn order to determinate the affect of coptis alkaloids on the H2O2content of free radicals intracellular, the generation of reactive oxygen species, activity of SOD and expression of UCP2were measured Mitochondrial antioxidant, cellular antioxidant and immune protein blotting experiments were carried out.(1) The effect on expression of UCP2showed that coptisine can promote the expression of UCP2to maximum extent, followed by berberine, palmatine and epiberberine effect is not obvious.(2) Antioxidant activity:four alkaloids could cleared H2O2free radicals in mitochondria in vitro and inhibit the production of reactive oxygen species intracellular, suggesting that four alkaloids have the ability of antioxidant. It showed that Rhizoma coptidis alkaloid can promote the expression of UCP2and inhibit the generation of ROS.2The expression of UCP2and antioxidant activity of coptis alkaloids in cells: Hyperglycemia rats were treated with coptis alkaloids to measure the rat body weight, the expression of UCP2and the activity of SOD enzyme in rat serum after four-months-feed. The results showed that alkaloids hardly influenced on SOD activity, but promoted the expression of UCP2obviously.