| Parkinson’s Disease（PD）is a kind of nervous system degenerative disease affectingabout one percentage of people over55years old. Deep Brain Stimulation (DBS) techniquehas been widely applied to the treatment of PD, however, many aspects about this treatmentmethod is still controversial.This topic utilized stereotaxic technique to inject6-hydroxydopamine (6-OHDA) intothe ventra tegmental area (VTA) and substantia nigra pars compacta (SNc) of the rats to buildup PD animal models. Self-made electrode was implanted into PD rats’ brain, local fieldpotentials (LFPs) signals of internal segment of globus pallidus (IGP） was recorded aftergiving DBS to the parafascicular nucleus (PF). And then recording the LFPs of the PF aftergiving electrical stimulation to IGP. Meanwhile, the LFPs of the PF and IGP were recorded ofnormal rats after implanting the electrodes into the normal rats.Spectral analysis for the LFPs using Matlab software revealed that IGP LFPs of PD ratscompared with that of normal rats changed a lot, which assumed that the percentage of IGPLFPs in the frequency range of below30Hz of total signals was extremely decreasing, thepercentage of IGP LFPs in the frequency range of above30Hz was extremely increasing. Thepercentage of PF LFPs of PD rats, compared with that of normal rats, in the frequency range ofbelow7Hz of total signals was extremely decreasing, the percentage of PF LFPs in thefrequency range of from7Hz to30Hz was unchanged, the percentage of PF LFPs in thefrequency range of above30Hz was also extremely increasing. Power spectral density (PSD）analysis resulted in that the power value change of IGP and PF LFPs of experimental ratscompared with that of normal rats conformed to the result of spectral analysis.Spectral analysis for the effects of DBS revealed that the percentage of IGP LFPs of PDrats in the frequency ranging from30Hz to100Hz of total signals was obviously increasing,after giving DBS to PF, and the percentage of IGP LFPs of the other frequency was unchanged.The percentage of PF LFPs of PD rats in the frequency range of below30Hz of total signalswas extremely decreasing after giving DBS to IGP, and the percentage of PF LFPs of the other frequency was extremely increasing. PSD analysis resulted in that the power value of IGPLFPs of PD rats in the frequency range of above25Hz was obviously enhanced after givingstimulation to PF. The change of power value of PF LFPs of PD rats after giving stimulation toIGP were the power of frequency above20Hz was also enhanced.The results of neutral red staining and immunohistochemistry were that the damaged sideof compacta zona of the substantia nigra (SN) in the PD rats was obviously disappeared, andcompared with the normal side, soma of neuron became shriveled and there was almost noobvious neuron structure. Utilizing the method of immunohistochemistry to deal with the brainslice of PD rats after separately stimulating to PF and IGP showed that the degree of injury ofthe damaged side of SN was hardly improved.This topic can be concluded that both PF and IGP LFPs of PD rats changed comparedwith normal rats, and the firing changes of the two nucleus showed that percentage of signalsof low frequency decreased, and that of the high frequency increased. DBS to PF madepercentage of IGP LFPs of high frequency increase and the power value of the high frequencysignals also increase. It declared that the stimulation could enhance the firing activity of IGP.The influence of DBS of IGP to PF was that percentage of PF LFPs of low frequencydecreased and percentage of signals of high frequency increased, and power value of the highfrequency signals also increased. It also declared that the stimulation could enhance the firingactivity of IGP. Meanwhile, from the effect of stimulation conclusion can be given: function ofstimulating of IGP to PF was stronger than that of stimulating of PF to IGP, and LFPs ofnucleus in PD rats could be changed through stimulation, then provided reference to the choiceof the clinical DBS targets in the field of basical research. Meanwhile, this topic can alsoprovide reference value for the mechanism of DBS after stimulating through usingimmunohistochemistry to observe the change of DA neurons in SN after the DBS. |
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