Correlation Of Steroid Receptor Coactivator-3and Trefoil Factor3Expression In Gastric Cancer And Precancerous Lesions With Angiogenesis

Background:Gastric carcinoma is a common human malignancy worldwide threatening to human health. Patients with early gastric cancer may be asymptomatic or only with nonspecific dyspepsia performance, which fail to diagnosticate. Bleeding, obstruction, abdominal mass or transfer symptoms may appear in advanced gastric cancer patients. At present, most of patients who recept clinical treatments are at the stage of advanced gastric cancer and the clinical outcomes are unsatisfactory. So looking for new markers for early diagnosis of gastric cancer can improve the detection rate of gastric cancer and prognosis.Objective:To investigate the expression of steroid receptor coactivator-3(SRC-3) and trefoil factor3(TFF3) in gastric carcinoma and precancerous lesions, and explore their correlation with microvessel density (MVD) in gastric carcinogenesis and angiogenesis.Methods:The expression of SRC-3and TFF3was detected by the immunohistochemical method in26cases of superficial gastritis,25cases of gastritis with intestinal metaplasia,20cases of atypical hyperplasia and66cases of gastric carcinoma, which were confirmed by pathology. MVD was detected by immunohistochemistry using anti-CD34antibody.Results:①The expression of SRC-3was gradually increased with superficial gastritis, intestinal metaplasia, atypical hyperplasia and gastric cancer(3.8%、28.0%、45.0%、51.5%, respectively). The expression of SRC-3was higher in gastritis with intestinal metaplasia, atypical hyperplasia and gastric cancer than in superficial gastritis (P<0.05; P<0.01; P<0.01). The expression of SRC-3was higher in gastric cancer than in gastritis with intestinal metaplasia(P<0.05). There was no significant difference in SRC-3expression between gastritis with intestinal metaplasia and atypical hyperplasia,as well as atypical hyperplasia and gastric cancer (P>0.05).②The expression of TFF3was7.7%in superficial gastritis,60.0%in intestinal metaplasia,65.0%in atypical hyperplasia and56.1%in gastric cancer. The expression of TFF3was significant higher in gastritis with intestinal metaplasia, atypical hyperplasia and gastric cancer than in superficial gastritis(P<0.01, respectively).The expression of TFF3was lower in gastric cancer than in gastritis with intestinal metaplasia, and atypical hyperplasia. There was no significant difference in TFF3expression among three groups(P>0.05).③MVD was gradually increased with superficial gastritis, intestinal metaplasia, atypical hyperplasia and gastric cancer (17.45±2.52、23.24±1.72、24.36±5.22、32.44±3.92, respectively). MVD was higher in gastritis with intestinal metaplasia, atypical hyperplasia and gastric cancer than in superficial gastritis (P<0.01, respectively). MVD was higher in gastric cancer than in gastritis with intestinal metaplasia, and atypical hyperplasia (P<0.01,respectively). There was no significant difference in MVD between gastritis with intestinal metaplasia and atypical hyperplasia(P>0.05).④The expressions of SRC-3and TFF3in gastric carcinoma were correlated with lymph node metastasis and TNM staging (P<0.05, respectively), not associated with patients’ age, gender, tumor size, degree of differentiation, invasion depth (P all>0.05). MVD was correlated with lymph node metastasis,TNM staging and depth of tumor invasion(P<0.01, respectively), not associated with patients’ age, gender, tumor size, degree of differentiation (P all>0.05).⑤MVD with positive expressions of SRC-3、TFF3were higher than those with negative expressions (P<0.01, respectively). The expressions of SRC-3and TFF3were positively correlated with MVD(r1=0.434,r2=0.672).Conclusion:SRC-3、TFF3and CD34were over-expression in gastric carcinogenesis and development, which may play important roles in carcinogenesis, invasion, metastasis and angiogenesis. They can be used as a useful indicator of the early prediction and prognosis of gastric cancer.