| The complement system plays an important role in host immune defense againstinfection and in the clearance of antigen-antibody complexes from the bloodstream.However, when activated inappropriately, the complement system may cause a variety ofdiseases such as system lupus erythematosus (SLE), rheumatoid arthritis (RA), and acuterespiratory distress syndrome (ARDS). With the increases in our understanding of the roleof complement activation that involved in the diversified pathological progresses, theinhibition of complement activation in these diseases or surgical applications would likelyhave significant benefits. But there is still no desirable therapeutic compound available onthe market for complement inhibition. Numerous naturally occurring agents have beenshown to have anti-complementary effects, and they offer the prospect of inexpensive andnon-toxic strategies for the therapeutic inhibition of complement. As part of our continuouseffort to search for anti-complementary agents from the spikes of Rhodiolasachalinensis,bioactivity-directed fractionation and isolation of these plants were carriedout,32compounds were isolated and identified.The optimal dilutions of sera (resources of complements) that gave submaximal in theabsence of complement inhibitors were determined to perfect the hemolysis assay todetermine different extracts of Rhodiola sachalinensis，The results showed that EtOAcextract (CH50=0.58±0.03mg/ml) and n-BuOH extract (CH50=0.48±0.02mg/ml) weremore active than its petroleum extract(CH50=1.75±0.08mg/ml).Rhodiola sachalinensis A.Bor. belongs to the plant family Crassulaceace. All the partsof the plant can be used as herb medicine, In recent years, researchers at home and abroadhad found many components from the plant, including penylpropanoids and flavonoidcompounds, inaddition terpenoids, organic acid, polysaccharide,and other compounds.Modern pharmacological studies had shown that Rhodiola L.plant had a lot ofpharmacological activities, such as anti-cancer, anti-virus, anti-fatigue, nootropic,life-span and immune regulation.During the course of our investigation for bioactive agents from the EtOAc extractand n-BuOH extract of roots and stems of Rhodiola sachalinensis,32compounds wereobtained by using silica gel column chromatography, Sephadex LH-20chromatography,and Semi-HPLC, and structures of32compounds were elucidated through spectroscopicanalysis（NMR, MS and IR）.Their structures were identified to be gallic acid (HJT-1),p-hydroxybenzoic acid (HJT-2), kaempferol (HJT-3), kaempferol-3-O-β-D-glucopyranoside (HJT-4), kaemnpferol-3-α-L-rhamnoside (HJT-5), kaempferol-7-O-β-D-glucopyranoside (HJT-6), kaemnpferol-7-α-L-rhamnopyranoside (HJT-7), kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside (HJT-8), Leucoside (HJT-9),kaempferol-3-O-β-xylopy ranosyl(1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside(HJT-10), Herbacertin-3-O-β-D-glucopyranoside (HJT-11), Herbacertin-7-O-β-D-glucopyranoside (HJT-12), Herbacertin-7-α-L-rhamnopyranoside (HJT-13), Herbacertin-3-O-β-D-glucopyranosyl-7-O-α-L-rhamnopyranoside (HJT-14), Rhodiosin (HJT-15),Herbacertin-3-O-β-D-glucopyranosyl-7-O-β-D-glucopyranosyl(1→3)-7-α-L-rhamnopyranoside (HJT-16), Herbacertin-3-O-[α-L-rhamnopyranosyl(1→2)-β-D-glucopyranosyl]-7-O-α-L-rhamnopyranoside (HJT-17), quercetin (HJT-18), quercetin-3-O-β-D-glucopyranoside (HJT-19), Tricin (HJT-20), Tamarixetin (HJT-21), Rhodiolinin (HJT-22),Cinnamyl-O-β-D-glucopyranodide (HJT-23),Cinnamyl-(6ˊ-O-β-D-xylcopyranosyl)-O-β-D-glucopyranoside (HJT-24), Rosovin(HJT-25), Rosarin (HJT-26), Benzyl-O-β-D-glucopyranodide (HJT-27),Phenylethyl-O-β-D-glucopyranodide (HJT-28), Phenylpropyl-O-β-D-glucopyranodide(HJT-29), Salidroside (HJT-30), Sachalinol (HJT-31), Rosiridin (HJT-32).Compound HJT-6,HJT-19and HJT-21were obtanined from the plant in this genusfor the first time.Compound HJT-2, HJT-8, HJT-11, HJT-12,HJT-14,HJT-18andHJT-31were obtained from this plant for the first time.Among these compounds,24compounds including flavonoids,phenolic andsalidroside showed anti-complement activity. |
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