Association Of Myeloid-derived Suppressor Cells,Interleukin-10and Interleukin-12with Wheezing Infants

For infancy and early childhood,wheezing is common respiratory symptoms.Wheezing disease is a group of wheezing respiratory syndrome in children.because of age-specific pathophysiological characteristics of wheezing in infants and young children,Wheezing often occur more than once. Some of them are the early onset of asthma. But the pathogenesis of infants wheezing is not clear. Determining temporary wheezing or asthma of wheezing infants is still a problem in the pediatric clinical work.PurposeInvestigate the accumulation of myeloid-derived suppressor cells (MDSCs) and Interleukin-10(IL-10), Interleukin-12(IL-12) in children with wheezing or bronchiolitis children to understand their possible significance,in order to explore their clinical significance in infants wheezing.Materials and methodsThe patients subjects were firstly categorized into four groups:bronchiolitis group,wheezing group, pneumonia group and non-infectious group. The bronchiolitis group consisted of99children (51males,54females) aged from3months to2years (ave=l years and6months)with bronchiolitis,who visited a doctor or were hospitalized in the Third Affiliated Hospital of Zhengzhou University from October,2010to June,2012, With at least one risk factor for children52cases as bronchiolitis group I, no risk factors in children with47cases as bronchiolitis group II (atopic risk factors means:children of their own, including a doctor’s diagnosis of allergic dermatitis, allergic rhinitis and other allergic diseases, or one or both parents suffer from, a history of the asthma disease). the children who have≥2times wheezing in six months or≥3times wheezing in12months were considered as recurrent wheezing.The wheezing group consisted of103children (51males,52females),aged from7months to2years and lmonth(ave=lyears and7months) with recurrent wheezing, who visited a doctor or were hospitalized in the same time period,The wheezing group was divided into two subgroups:wheezing group I (The asthma predictive index positive)(for50cases)and wheezing group II (The asthma predictive index negative group)(for53cases),who were confirmed according to the asthma predictive index standards in " Guideline for Diagnosis and Treatment of Asthma in Childen ".The non-infectious group comprised of54non-infectious children (28males,26females)aged from6months to2years and4month(ave=lyears and7months)randomly chosen from those who had surgical hernia,kidney stones or other non-infectious diseases in the hospital in the same time period. The pneumonia group comprised of50children (25males,25females) aged from6months to2years and3months(ave=l years and8months),who were diagnosed with pneumonia in the same hospital in the same time period.Each group of children age and gender differences were not statistically significant (Pa>0.05), each group of children were born full-term, excluding tumors, airway malformations in children, nearly two weeks without infection, no history immunomodulatory agents to use, All work was consistent with parents and the requirement of the Hospital Ethics Committees.MDSCs in peripheral blood mononuclear cells were detected with flow cytometry.The plasma concentration of IL-10,IL-12were detected with enzyme-inked immonosorbent assay(ELISA)SPSS17.0statistical software was use for statistical analysis。Each group of measurement data were expressed as mean±standard(X±S) deviation。The differences between groups were analyzed using one-way ANOVA, Bonferroni test, as a=0.05said the difference was statistically significant。Reslts1. The accumulation of MDSCs, serum IL-10in children with wheezing group I were significantly higher than those in wheezing group II (P<0.05);The accumulation of MDSCs, serum IL-10in children with wheezing group Ⅱ were significantly higher than those pneumonia and non-infectious group (P<0.05), however, the accumulation of serum IL-12in children with wheezing group I were significantly lower than those in wheezing group II, pneumonia and non-infectious group (P<0.05);2. The accumulation of MDSCs, serum IL-10in children with bronchiolitis group I were significantly higher than those in bronchiolitis group II (P<0.05);The accumulation of MDSCs,serum IL-10in children with bronchiolitis group II were significantly higher than those in pneumonia and non-infectious group (P<0.05), however, the accumulation of serum IL-12in children with bronchiolitis group I were significantly lower than those in bronchiolitis group II.3. The accumulation of MDSCs, serum IL-10, IL-12did not show significantly difference between the children with wheezing group I and bronchiolitis group I (P>0.05).The accumulation of MDSCs, serum IL-10, IL-12did not show significantly difference between the children with wheezing group II and bronchiolitis group Ⅱ (P>0.05).4. The accumulation difference of MDSCs, serum IL-10, IL-12did not show significantly difference between children with pneumonia and non-infectious group;5. Correlation analysis:The accumulation of MDSCs in children with wheezing group I and bronchiolitis group Ⅰ was positively correlated with the accumulation of IL-10,however,negatively correlated with the accumulation of IL-12. ConclusionsMDSCs may play a very important role in the pathogenesis of asthma after bronchiolitis and recurrent wheezing by up-regulating the accumulation of IL-10and down-regulating the accumulation of IL-12.