Effect Of Yishentongluo Decotion In The Expression Of Transforming Growth Factor And Type â…£ Collagen In Kidney Tissue On Membranons Nephropathy Model Rats

Objective:Experimental study was to analyze the effect of YishentongluoDecotion in the expression of24hours urine protein, blood biochemistry, renalpathology, ColⅣmRNA and TGF-β1mRNA in renal tissue on membranonsnephropathy (MN) rats. The probable pathogenesis of MN and the therapeuticvalue of Yishentongluo Decoti in MN were investigated, so as to provide thenew therapies and effective drugs for MN.Methods:60healthy male Wistar rats with body weights at200±20gwere randomly divided into two groups: Normal group is15, and Modelinggroup is45. According to the way of establishing MN rats, Modeling groupmulti-site injected cationic bovine serum albumin (C-BSA) in underarm andinguen once every other day for preimmunization, then established MN ratmodal after one week through the way of injecting C-BSA3times a week for4weeks. After the successful establishment of MN model, the rats ofmodeling groups were randomly divided into three groups: Modal groups,Yishentongluo Decotion group and Benazepril group. The two treatmentgroups via oral administration corresponding drug for4weeks, the othergroups via oral administration same amount of distilled water. Each group wasfree to move, eat and drink. Each group collected the24h urine at metabolismcage at three time points: before modeling (at the end of first week), aftermodeling (at the end of sixth week), after treatment (at the end of tenth week).We take the blood in abdominal aorta at the end of tenth week. The levels ofALB, TP, TC, TG, Scr and BUN in serum were assayed. Then the renal tissuewas collected. Pathologic changes of renal tissue were observed with electronmicroscope, light microscope and immuofluorescence. Expression ofTGF-β1mRNA, Col Ⅵm RNA in renal tissue was observed with real-time PCR. Results:124h urinary protein quantitative analysis showed thatCompared with Normal group, the other groups manifested severeproteinuria after injecting with C-BSA for four weeks. There was a significantdifference (P<0.05). Compared with Modal group, the24h urinary proteinquantitative analysis of Yishentongluo Decoti group, Benazepril groupdeclined obviously after treatment. There was a significant difference(P<0.05); There wasn’t a significant difference between Yishentongluo Decotigroup and Benazepril group (P>0.05). After treatment, the24h urinary proteinin two treatment groups was significantly different from pre-treatment(P<0.05).2After four weeks treatment, serum biochemistries analysis showed thatWhen the treatment concluded, the serum TP, ALB of YishentongluoDecoti group, Benazepril group and Modal group declined at different degrees.There was a significant difference with that of Normal group (P<0.05); Theserum TP, ALB of Yishentongluo Decoti group, Benazepril group elevatedmarkedly. There was a significant difference with that of modal group(P<0.05), but there wasn’t a significant difference between Benazepril groupand Yishentongluo Decoti group (P>0.05); The serum TC, TG ofYishentongluo Decoti group, Benazepril group, Modal group elevated atdifferent degrees. There was a significant difference with that of Normal group(P<0.05); The serum TC, TG of Yishentongluo Decoti group, Benazeprilgroup declined obviously. There was a significant difference with that ofModal group (P<0.05), but there wasn’t a significant difference betweenBenazepril group and Yishentongluo Decoti group (P>0.05); The serum BUN,Scr of all rats hadn’t a significant difference (P>0.05).3Renal tissue of rats was observed with light microscope, electronmicroscope and immuno fluorescenceThe light microscope observation result showed that: The structure ofglomerulus in Normal group was normal. In Modal group, the volume ofglomerulus enlarged markedly; Cells increased in glomerulus; a lot of inflammatory cells infiltrated into glomerulus; PASM-HE staining showed thatGBM thickening and subepithelial deposition; Renal interstitium could see alittle lymph and mononuclear cell infiltration, fibrosis, wall thickening ofsmall arteries. In two treatment groups glomerulus was enlarged slightly,GBM wasn’t thickening obvious, a little subepithelial deposition could beobserved.The electron microscope observation result showed that: In Normal group,GBM was not thickening, the electron dense deposit wasn’t seen insubepithelial, foot process wasn’t fusion. In Modal group, GBM showirregular thickening, epithelial side had quantity of electron dense deposit,diffuse foot process was fusion and disappearance. In two treatment groups,GBM was thickening slightly. GBM had a little electron dense deposit, diffusefoot process was fusion partly.The immunofluorescence observation result showed that: There wasn’tsignificant abnormality in Normal group, Fluorescent display strong (－); InModal group, rough granular lgG and C3distributed throughout the capillaryloops of the glomerulus to various degree. Fluorescent display strengthen,about (+++~++++). In Yishentongluo Decoti group, a little fine granular lgGand C3distributed throughout the capillary loops of the glomerulus, about(+~++).4Real-time PCR result analysisWhen the treatment concluded, the expression of TGF-β1mRNA,Col ⅣmRNA in Yishentongluo Decoti group, Benazepril group and Modalgroup elevated to various degree, there was a significant difference with thatof Normal group(P<0.05). The expression of TGF-β1mRNA, ColⅣ mRNA inYishentongluo Decoti group, Benazepril group declined to various degree,there was a significant difference with that of Modal group (P<0.05). Trerewas no significant difference between Benazepril group and YishentongluoDecoti group (P>0.05).Conclusions:1According to many yeas clinical experience, our tutor proposed Yishentongluo Decoti. The method of treatment was “strengthening spleen andkidney, promoting blood circulation for removing obstruction in collaterals”.And it was based on MN asthenia in origin and asthenia in superficiality. Andexperimental study showed that: Yishentongluo Decoti was effective treatmentfor that.2The experimental study showed that: Yishentongluo Decoti couldreduce MN rats’24h Urine protein, improve TP and ALB in plasma, reduceTC and TG in plasma, alleviates the MN progress.3Renal pathology showed that: Yishentongluo Decoti could reduceimmune complexes deposition in MN rats’ GBM, control GBM thickening,and improve the injury in MN rats’ kidney to some degrees. YishentongluoDecoti could control the expression of TGF-β1mRNA and ColⅣmRNA innephridial tissue, so as to provide good protection on MN rats’ kidney.