Studies On The Effect Of Anticancer By MnSOD Gene-armed Oncolytic Poxvirus

Into the21st century, with the life and work increasing rapidly, continuously improve thechance of human suffering from cancer and other major diseases, cancer, has replacedcerebrovascular diseases seriously harm and threat to human health and become The first killer ofhuman life and one of the world’s largest public health problem. Therefore, overcome cancer hasbecome urgent task of human.Biological therapy of cancer, from gene therapy to virus therapy and targetinggene-virotherapy Strategy has proposed. More and more people applaud it.However, clinical trialdata from many years indicated that they were relatively safe and selective for cancer, buttherapeutic potency was limited after both direct intratumoral but the effect of anti-tumor did notreach the expected results. Because the vector systems of gene therapy can neither specifictransfection all tumor cells nor can the therapeutic gene efficient expression in the tumor in thehuman body. So the biological effects of the therapeutic gene is difficult to exert effect and theoccurrence of tumors is a multi-step process that includes a variety of genetically modified, thus cannot completely eliminate the tumor.Vaccinia and other poxviruses have widely host range, for choice,large genome canaccommodate the large fragment of exogenous DNA, suitable for expresses many genes.itsimmunogenicity can enhance the host immune destruction of the tumor they can travel systemicallythrough the blood, Vaccinia virus can targeted transport to tumor tissue. Recombinant vaccinia virushas deleted TK can selectively replicate with in and lyse cancer cells, so the purpose gene can bepreferably expression in the local of tumor. Thus the Vaccinia virus is an ideal gene therapy vectorin gene therapy of cancer and will have a promissing prospects in cancer gene.MnSOD is a novel tumor suppressor gene, can prevent normal cells canceration, inhibit thegrowth of tumor, and enhance tumor cells apoptosis, it is a novel and effective gene for cancertherapy.In thisr Study Effect of Anticancer by MnSOD Gene-armed oncolytic Poxvirus whichmanganese superoxide dismutase MnSOD gene insert into TK gene of vaccinia virus in vitro.the results showed that the MnSOD gene-armed oncolytic Poxvirus OncoPox-MnSOD and controlvirus OncoPox-EGFP have significantly killing effect on cancer cel and no toxicity to normal cells. Western blot analysis showed that MnSOD can effective expression and induced apoptosis after theOncoPox-MnSOD infected cells.