Yellow Ball Spore Raw Fiber Slime Mold Enzyme System Characterization Of Autophagy And Its Degradation Products Were Analyzed

With the rapid development of society and economy, the demand of fossil energy is increasing, and the shortage of energy is becoming a major issue which will hinder the development of human society. Meanwhile, biomass, the most abundant renewable resources on the earth, has not been effectively utilized. Biomass can replace fossil energy totally and become a reliable clean energy for human beings, however, as a result of the heterogeneity of substrate structure and existence of antidegradation barrier, research related with biomass conversion develop slowly. The biomass degradation efficency of mcirobial community in nature environment is relatively high, which provides a good reference to convert biomass into energy that is more convenient to use.Sporocytophagamyxcococcoides is widely preads in soil and has rather high degardation ability to lignocellulose biomass; it can also form cysts when the condition is not suitalbe for growth. Therefore, it is a great practical significance that studying its degradation strategy thereby improving the biomass degradation efficency It can also secrete plenty of extracellular polysaccharide while degrading lignocellulose, which has a potential industrial applicaetion. This article starts from the identification and cultivation of Sp. Myxcococcoides, and then concentrates on the researchs of its enzyme system, including the location and properties of its enzyme system, analysis of the ultrastructure of degradation substrate as well as degradation products, the main work and results are as follows:Through16S rRNA molecular biology identification, microbial physiological and biochemical and ultrastructure analysis, the speices of bacterial strains keep in laboratory is determined to be Sp. Myxcococcoides. It can grow on medium with glucose, cellobiose, sucrose, raffinose as carbon resourse, but there are no extracellular cellulose enzymes. It cannot grow on medium with xylose, xylan, arabinose; It can also grow fast on the medium with crystalline cellulose as sole carbon resourse while secrete small amounts of4kinds of endoglueanases and2kinds of xylanases. The bacteria will grow faster when the particle sizes and crystallinity is lower. The optical concentration of crystalline cellulose for liquid fermentation is0.5%, bacteria in exponential growth period can untilize90%of cellulose in24hours. Although the extracellular enzyme activity is limited, the bacteria can degrade crystalline cellulose efficiently. Obeservation of sbustrate ultarstructure has showed that thallus palys a key role in the rapid degradation of crystalline cellulose. Lignocellulose degradation enzymes are also found on the outer membrane, however, enzyme system properties have showed that dissociated outer membrane enzymes degradation efficiency is low, which means their activities are not enough for rapid degradationThe hydrolyzate analysis of Sp. Myxcococcoides has showed that there are extracellular transglycosylation related enzymes, which can convert cellobiose into cellotriose and provide the material basis for the synthesis of the extracellular mucopolysaccharides. The extracellular enzymes or the outer membrane proteins cannot degrade crystalline cellulose, which has confirmed the improtance of the thallus itself in degradation process again Although it can not grow on the substrate such as xylose and xylan, its enzyme systems can degrade xylan into xylooligosaccharides, and maybe the purpose of this strategy is to improve the accessibility of cellulose.