Mountain Bean Root Polysaccharide Extraction Process And Antitumor Activity Of Study

Radix sophorae tonkinensis is the roots and stem of Leguminous plants of the genus tree inVietnam Sophora japonica. It is widely recognized that Sophora subprostrate plays multiple rolesin pharmacological and biological function as a traditional Chinese herbal medicine, and its hascertain antitumor activity. The Master’s thesis was carried out to extract and separatepolysaccharides from Sophora subprostrate, optimize the extractive technology and study theantitumor activity and its mechanism of through the experiment of inhibiting tumor in vitrodirectly lethal effect to tumor cell. The thesis include four chapters and each chapter follows:The first chapter: Literature Review. Polysaccharides from sohpara subprastrat. are the mainsupporting matrix of the edible sohpara subprastrat. and key bioactive components. They have anumber of bioactivities such as immunomodulation, anticancer and autoxidation. This paperoverviews the extraction technology, the methods of isolation and purification, the chemicalstructure and the physiological activity in the polysaccharides from the roots of Sophorasubprostrata.. The paper also summarized the research results of animal test in recent year andprospected the main research of the polysaccharides form sohpara subprastrat..The second chapter: The extraction and separation of polysaccharides from sohparasubprastrat. This chapter optimizes the extraction technology of polysaccharides from SophoraTonkinensis Gapnep. Orthogonal experimental design was used to select optimum technology ofPolysaccharides extraction from Sophora Tonkinensis Gapnep. and the content of totalpolysaccharides were determinated by Phenol-vitriolic Colorimetry. Optional extractiontechnology of Polysaccharide was as follow: Solid-liquid ratio1:10; extraction time90min;extraction two times. The content of polysaccharide is3.62%. The method was simpleaccurate sensitive and suitable for extraction polysaccharide from Sophora Tonkinensis Gapnep.The Third chapter: Identify the polysaccharides from sohpara subprastrat.. In this chapter, thecontent of total polysaccharides was determinated by Phenol-vitriolic Colorimetry and featuresgroups were characterized by Infrared Spectrophotometer.The fourth chapter: Antitumor activity experiments of polysaccharides from sohparasubprastrat.. This chapter study the antitumor activity by microfluidic chip, on which integrate aconcentration gradient generator and cells culture units. MTT experiments prove that a certain concentration of polysaccharides from sohpara subprastrat. can restrain SMMC-7721liver cancercells and EC109esophageal cells. Colonyforming Assay experimental results show that thepolysaccharides from sohpara subprastrat. can effectively restrain the colony forming ofSMMC-7721liver cancer cells. A flow cytometry technology demonstrate that the polysaccharidesfrom sohpara subprastrat. can induce the liver cancer SMMC-7721early apoptosis.