Cadmium Treatment On Artemisinin Has Hormesis Effects Of Preliminary Discussion

This study, controlled trials to cultivate Artemisia annua Las test material, through hydroponics processing and spraying treatment determined stimulation of different concentrations of cadmium, Artemisia annua L. biomass accumulation, photosynthesis, secondary metabolite biosynthesis and other indicators to explore the different treatment way heavy metal cadmium on the physiology and biochemistry in Artemisia annua L. The main contents include:1.An artificial gastrointestinal fluid simulated digestion and movement, examines the impact of heavy metal content of wild Artemisia annua L. in Guangdong, Jilin, Guizhou provinces on human health;2. Artemisia annua L. biomass by measuring the spraying different concentrations of cadmium accumulation, photosynthesis, the artemisinin content, exploring spraying Cd concentration range of Hormesis effect on Artemisia annua L.3.Measurements of different concentrations of Cd in the nutrient solution on Artemisia annua L. antioxidant enzymes CAT, POD activity, biomass accumulation, photosynthesis, the artemisinin content to explore the nutrient solution of cadmium treatment on Artemisia annua L. Hormesis effect phenomenon.Results:1.Guangdong, Jilin, Guizhou provinces, Artemisia annua L. fight powder using artificial gastrointestinal fluid simulation digestion, the use of electric hot plate digestion using ICP-MS detection of digestive juice known herbs in three provinces of Cu dissolution rate range of67.07%-82.86%Pb dissolution rate of the range of55.67-78.27%of As dissolution in the range of29.23-55.62%Cd dissolution rate in the range of59.32-85.42%, provincial herbs Hg dissolution rate of0.2.Cadmium spraying conditions of Artemisia annua L. aerial parts fresh weight impact in dealing with a short period of time does not appear significant change in trend, and processing to14d when treated with20μmol/l concentration group and100μmol/l. Concentration group was significantly higher, the underground sections fresh weight for14days showed the same trend; Artemisia annua L. root length of the longest in the handling of100μmol/l, significantly higher than the control group and other treatments; the number of leaves treated with20μumol/l treatment group was significantly higher, but other treatments there were no significant differences; other biomass parameters were not changed significantly with the concentration and processing time.The initial stress, chlorophyll a and b content was not obvious, when processing9d control group chlorophyll a and b content is lower than the different concentrations of cadmium treatment group compared with the processing6d, a declining trend, to14d, chlorophyll a, b, content appeared to rise, less20,60μmol/l concentration group and control group, but100μmol/l was significantly lower than the control group. Thus, little spraying cadmium deal with short-term impact on chlorophyll synthesis, did not significantly inhibited, but with time, the inhibitory effect of high concentrations of chlorophyll synthesis is gradually emerging. Spraying cadmium treatment in a short period of time did not affect plant growth, with time, spraying processing plant fresh weight; annua fluorescence parameters in the low concentrations of cadmium treatment did not affect significantly with concentration, the fluorescence parameters first The trend of increased and decreased; a short period of time on the synthesis of chlorophyll, with time, the inhibitory effect of high concentrations of chlorophyll synthesis became clear.Artemisinin content in the treated with20μmol/l treatment group with changes in the processing time does not appear significant difference is not obvious to prove that low concentrations of sprayed cadmium on plant stimulation,60μmol/l treatment group with time, the artemisinin content decreased gradually, prove that when spraying cadmium concentration of60μmol/l, the synthesis process of the Artemisia annua L. artemisinin inhibit; when the concentration was100μmol/l, the artemisinin content in the processing6d increased, then the processing time extended, a gradually declining trend.3For8h, CAT activity increased with the concentration of cadmium appear first decreased and then an upward trend in the maximum60.222U/(g.min) in the treatment of100μmol/l; for Id, the20μmol/ICAT activity with the control group compared to no significant differences, other concentrations were lower than the control group; handle3d, CAT activity has a two-wave peak at60μmol/l and100μmol/l-32.265U/(g.min) and33.248U/(g min); deal with6d, CAT activity in treated with20μmol/l concentration at reduced13.794U/(g.min) to100μmol/l. Department increased to54.085U/(g.min); processing12d, CAT activity of the cadmium concentration trend is not obvious. For8h, POD activity is relatively stable trend with concentration, compared with the control group did not show significant differences in the processing1d, POD activity in60μmol/l concentration at the highest-413.187U/(g.min).Processing16d, Artemisia annua L. treated with20μmol/l treatment group on the ground, the underground part of the fresh weight compared with the control group does not appear significant difference compared to the other treatment group and control group are lower than the control group, the high concentration group was significantly lower than control group, indicating that the processing time of384h, Artemisia annua L on the ground, underground part of the fresh weight with the increase of cadmium concentration decreases at the same time can see the impact of cadmium on plant roots; plant height in the high concentration of60μmol/l,80μmol/l,100μmol/l,120μmol/l treatment group was significantly lower than the control group, low concentrations treated with20μmol/l,40μmol/l treatment group compared with control group there were no significant differences; root length and plant height, the same trend, with the cadmium concentration increases, decreases gradually treated with20μmol/l,40μmol/l group and control group compared there were no significant differences; plant stem diameter did not show a clear trend; the number of leaves in the control group were greater than the treatment group, and60μmol/l80μmol/l,100μmol/l,120μmol/l. treatment groups significantly different.Processing1d, the artemisinin content of each treatment group differences,120μmol/l treatment group was significantly higher than other treatments, the content of60,40μmol/l treatment group second only to120μmol/l, significantly higher than0,20,80100μmol/l treatment groups, and between the four groups did not show significant differences; processing4d, the highest cadmium content of40μmol/l treatment group, but compared to the control group did not show significant differences,120μmol/l treatment group artemisinin content of the lowest and significantly lower than the other treatment group and control group; processing8d,60μmol/l treated with cadmium content in the highest compared with the control group did not show significant differences,80μmol/l treated with cadmium content the lowest, significantly lower than the other control group and treatment group, the20,40,100,120μmol/l treatment group, the artemisinin cadmium levels were lower than the control group, but did not show significant differences; processing16d, The artemisinin content of the treated group were higher than that in the control group treated with120μmol/l,40,60μmol/l treated with cadmium levels were significantly higher than the control and treatment groups.Conclusion:1.This result showed that Artemisia annua Pb and As, Guangdong Province exceeded the standard, and heavy metal content of the other two provinces within a limited range, can be seen that the different origin of Artemisia annua L. of heavy metals. Can be seen that the dissolution in the gastrointestinal fluid from three origin annua heavy metal, heavy metal dissolution less artificial gastrointestinal fluid was absorbed by the body lower heavy metal content does not pose a threat to human health.2.Cadmium stimulated the accumulation of artemisinin in Artemisia Hormesis effect. 3.Hormesis effect of artemisinin accumulation of cadmium stimulated time and concentration on the interaction.