| With the rapid development of industry and agriculture, various chemical pollutants being sustained discharged into the environment, and do not made a scientific and reasonable supervision and management. These substances will cause harm to the endocrine system, immune system, nervous system, reproductive system and other health problems for humans and all kinds of organisms. Thus, establishing and improving a sound environment safety standards and inspection detection system is a very significant. The matrix in the environmental estrogens are very complex, concentration of low, persist for a long time and other characteristic, which are susceptibly interfered to detected. So the pre-treatment methods will become important step when detection and analysis of environmental estrogen. Magnetic molecular imprinted polymers (M-MIPs) are obtained by magnetic nanoparticles combined with molecular imprinting technique. It is noteworthy that the M-MIPs have not only excellent superparamagnetism of the magnetic nanoparticles, but also have high selectively recognized the analytes in the complex matrix. The M-MIP will be a convenient and quickly recognizable separation method with external magnetic field. And the M-MIPs are provided a bran-new thinking and method for sample pretreatment.1. This thesis reviewed the current situation of the environmental estrogen, described a synthetic method and principle of the molecular imprinting technique. Its applications in solid phase extraction, chromatographic separation, sensors and so on were also summarized. Moreover, it was focus on magnetic molecularly imprinted materials, fluorescence functional material and its application materials.2. Magnetic Fe3O4nanoparticles were prepared by coprecipitation and then coated with SiO2on the surface. Fe3O4@SiO2composite microspheres were modified by KH570. Using molecular imprinting technology, atrazine magnetic molecularly imprinted polymer was prepared by using atrazine as template molecule, methacrylic acid as functional monomer and ethylene glycol dimethacrylate as cross-linkers. The morphology, composition, thermal performance, crystal structure and magnetic properties of magnetic nanoparticles were characterized. The recognition selectivity of polymer was studied for template molecule and simulation by UV spectrophotometry. The adsorption properties and selectivity ability were analyzed by Scatchard analysis. Scatchard linear regression analysis indicated that there are two binding sites of the target molecules. The magnetic molecularly imprinted polymer has been applied to the analysis of atrazine in real samples. The results show that:the recovery rates and the relative standard deviation were94.0%～98.7% and2.1%～4.0%in corn; the recovery rates and the relative standard deviation were88.7%～93.5%and2.8%～7.2% in water.3. The magnetic fluorescent nanoparticles were prepared by on the basis of the rare earth complexes of Eu and organic fluorescent element of fluorescein isothiocyanate as fluorescent material, and combined with Fe3O4@SiO2magnetic nanoparticles. Molecular imprinting technology was employed to produce one kind of bisphenol A magnetic fluorescent molecularly imprinted polymer microspheres (fluorescent M-MIP) by the surface of molecularly imprinted method using bisphenol A, methacrylic acid, ethylene glycol dimethacrylate, and the magnetic fluorescent nanoparticles as template molecular, functional monomer, cross-linking agent and imprinted supporter, respectively. The characterized of magnetic fluorescence molecularly imprinted polymer were used by fluorescence spectrophotometer, X-ray powder diffraction, vibration sample magnetic field meter, scanning electron microscope and transmission electron microscope. The recognition selectivity of magnetic fluorescence polymer (including M-MIP and M-NIP of fluorescence) was studied for template molecule and simulation. The results indicated that the fluorescent quenches of bisphenol A for fluorescent M-MIP were higher than that of the structural analogues, which illustrated selectivity recognition for bisphenol A of fluorescent M-MIP. Simultaneity, The fluorescent M-MIPs had much higher fluorescent quenches than the fluorescent M-NIPs at rebinding processes. |
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