E Yikui Ether Hydrochloride Pretreatment Of Hydrochloric Acid Caused By The Protective Effect Of Acute Lung Injury In Rats

Backgroud and ObjectivesAcute lung injury (ALI) is a kind of disease caused by the various lung insideand external pathogenic factors leading to acute and progressive respiratory failure. Itdevelops in patiens from a variety of clinical bad condition, including trauma, gastricaspiration, severe sepsis and shock. Further can develop for acute respiratory distresssyndrome(ARDS), which may cause extremely high mortality in critically patients(maybe forty-five to fifty percent). Its pathogenesis has not yet been fully understoodat present. The main pathological character of ALI is the development of interstitialpulmonary edema, rich in protein in the alveolar exudate owing to the increasing lungmicrovascular permeability and pulmonary hyaline membrane formation.Penehyclidine hydrochloride (PHC), a new kind of anticholinerigic drug, isindependently developed by Academy of Military Medical Sciences in the People’sRepublic of China. Its major character is the selectivity to M muscarinic receptor:PHC can selectively antagonize M1 and M3 receptor, and has no obvious antagonismon M2 receptor, which could obviate the tachycardia. Reserches indicated PHC canameliorate microcirculation, remit blood vessel permeability and reduce the release oflysosomal enzyme . Thus, PHC maybe have beneficial for the treatment ofALI..In this study we aimed to observe whether PHC has protective effect on ALIinduced by hydrochloric acid. We detected the expression of LXR- and TNF- inthe lung tissue of rats, and observed the effects of PHC on the expression of LXR- and TNF- in rats lung during development ofALI.MethodsThirty-two healthy SD rats of both sexes weighing 210-230 g were randomlydivided into 4 groups(n=8 each)：control group（group N）,ALI model group（groupALI）,intervention before the injury group(group P1, an hour before hydrochloricacid-induced in advance to PHC)and intervention after the injury group (group P2, anhour after hydrochloric acid -induced in advance to PHC ). The lung injury wasevaluated by using microscopy with hematoxylin and eosin(HE)staining and arterialblood gas. The TNF- content in the lung were detected by ELISA and expression of LXR- were detected by immuneo-histochemical staining.ResultLung injury in model group reached the pathologic criteria of acute lung injury.Hydrochloric acid increased TNF- content and decreased the expression of LXR- in lung compared with control group. In advance to PHC significantly ameliorated thelung injury in group P1, 2as compared to group ALI, and the effect of group P1isbetter than group P2.ConclusionPHC pretreatment can attenuate ALI induced by Hydrochloric acid throughincreasing the expression of LXR- in the lung.