Inverse Acupuncture Close Yuan Of Acupuncture Point Affect To Ovarian Hpo Axis In Rats

Objective:’Preventive acupuncture and moxibustion’mean to stimulate some points of the body by acupuncture or moxibustion in advance, which can improve the capability of defending diseases and supplementing’Zheng Qi’. And make the body have the function of preventing and alleviating the injury by the subsequent disease.’Preventive acupuncture and moxibustion’have better preventive function to the disorder of HPO axis.Peri-menopausal syndrome (PMS) is a series of syndromes, which is caused by the ovarian failure and disorder of the endocrine system, external metabolizing system, nervous system and psychic system of women before and after menopause. Clinical manifestations are menstrual disorder, hectic fever with sweating, burning sensation of hands and feet, fatigue, testiness, headache, vertigo, sonitus, cardiopalmus, forgetfulness, insomnia, and so on. To western medicine, the application of hormone replacement therapy is the first choice for PMS, however, it has all kinds of side effects. So, to find a safe and effective treatment increasingly become the research highlight. As an effective therapy for PMS without any side effect, Acupuncture attracts much attention. But there is less report about how to prevent PMS. And the application of acupoints is in shortage of evidence and objective criteria.By observing the regulatory effects of the preventive acupuncture and moxibustion at Guanyuan(RN4) points in ovariectomized rats. The research purpose of this study was to discuss the mechanism of’preventive acupuncture and moxibustion’on HPO axis.Methods:Experimental feminine SD rats were divided into5groups, including the normal group, fake operation group, ovariectomized rats group, preventive acupuncture group and preventive moxibustion group. ELISA was used to determine the levels of E2> ER、GnRH、FSH and LH in the serum and tissues including hypophysis、hypothalamus and uterus. The purpose of this study was to investigate the specificity of acupoints, estimate the regulatory effects of ’preventive acupuncture and moxibustion’on HPO axis, and also provide research evidences for acupoints selection and application in clinical practice.Results:1In comparison with the normal group, E2、ER and GnRH level in the tissue and serum of the model group was significantly lower(p<0.01), the levels of FSH and LH increased obviously(p<0.01).2Compared with the model group, E2、ER and GnRH level of the’preventive acupuncture’group and’preventive moxibustion’group increased in different degree (p<0.05); FSH and LH level reduced in different degree (p<0.05)3There is no obvious difference between the’preventive acupuncture’and ’preventive moxibustion’groups (p>0.05), except FSH of the blood,’preventive acupuncture’group is better than ’preventive moxibustion’group (p<0.01).Conclusions:1The’preventive acupuncture’and’preventive moxibustion’could modulate the lower estrogen level, and restrain elevated gonadotropin levels in ovariectomized rats, and has benign modulation to disordered HPO axis.2The ability of the’preventive acupuncture’and’preventive moxibustion’ are almost the same, except FSH of the blood,’preventive acupuncture’group is better than’preventive moxibustion’group.3The origination and development of PMS is closely involved with the HPO axis, and relates to multiple organs, such as the liver, spleen and kidney. The results of this study suggest that meridians, multiple organs, and both innate and acquired functions should be considered in clinical acupoints election. And we should put the emphasis on the crossing acupoints for prevention and treatment of PMS.