Brain Also Benefit Our Party For A Beta < Sub > 1-42 < / Sub > To Alzheimer's Disease Model Rats Learning And Memory, Cases Of Brain Tissue And The Influence Of The Tau Protein Phosphorylation

Purpose:To observe the changes in rat brain tissue to the hippocampal CA1region of condensed injection of AP1-42induced spatial memory and learning ability of rats, pathological changes in the brain tissue and tau protein phosphorylation protein kinase Aβ, GSK-3β, CDK-5expression change, to explore traditional chinese medicine-HNYCF of Aβ1-42induced by the effect and mechanism of learning and memory dysfunction.Methods:603months rats (Sprague Dawley, SD) are used in this study, the cerebral stereotactic injection to the rat hippocampal CA1region of condensed matter of Aβ1-42model of Alzheimer’s disease (AD). Two weeks later, when the operation was successful (pathological verification), according to results of Morris water maze and combining with weight,divided the rats into5groups randomly (SPSS software). Model group, the positive drug control drug (donepezil hydrochloride the piperazine homogeneous piece,0.49mg/kg) group, a small dose of the HNYCF (Beijing University of Chinese Medicine College preparation,3.54g of crude drug/g, and3.78g of crude drug/kg body weight)group, middle dose (7.56g of crude drug/kg) group, high-dose (18.90g crude drug/kg) group (n=12). Establish a separate group, the sham operation group (which the rat in hippocampal CA1region injection of same amount normal saline)(n=12). Dubbed the test drug to the equivalent solution, model group and sham operation group gavage the equivalent olume of water, once a day and continuous intragastric for12weeks. After12weeks,take the behavioral test of the water maze again, and then the rats were intraperitoneally anesthesia (Concentration of20%urethane solution, in accordance with0.7ml/100g dose), isolated the rat brain tissue, fixed with concentration of10%neutral buffered formalin, HE staining and Congo red staining of the pathology conventional chipper.Using a microscope to observe the changes in the structure of rat hippocampus. Measured by radioimmunoassay in hippocampus of amyloid-beta (beta-AP) and total protein content, using the immunohistochemical method to observe the expression of glycogen synthase kinase-3β (glycogen synthase kinase-3β, GSK-3p), β amyloid peptide (Aβ1-42), tau protein and cyclin-dependent kinase-5(of cdk-5) in hippocampal CA1region.Results:1、The results of Morris water maze show that, compared with the sham group, t he model caused by injected Aβ1-42to the brain of rats, the number of rats through the platform significantly reduced (P<0.05),time and distance in the fourth quadrant d ecreased significantly (P<0.05). The pathological results showed that:HE staining sho wed that the CA1region of model group were deeply stained, the staining of the cyt oplasm area is increase, the nuclei appeared pyknosis and deeply stained,cells arranged in a more scattered, free cells increased, but there was no obvious neurofibrillary tan gles; Congo red staining showed a higher amount of orange Aβ deposition in hippoca mpal CA1region, area of large and small,overall degree of staining deep, cells arrang ed in sparse and scattered, but did not find the formation of mature amyloid plaques. Immunohistochemistry results showed that:Compared with the sham group, expressio n of Ap\Tau protein, GSK-3β and CDK-5from model rats brain tissue was significant ly higher (P<0.01).2. After the gavage treatment of the three doses of HNYCF for12weeks, rats in the number of crossing of the plateau region, the time of the fourth quadrant and fourth quadrant of the journey has been an increasing trend. In this one, chinese medicine high dose group to a significant increase in rats in the fourth quadrant search time and distance (P<0.05, P<0.01). In the AD mode caused by Aβ1-42, the three dose groups of HNYCF, it have different inhibition in the expression levels of Aβ and hyperphosphorylation of Tau protein,GSK-3β and CDK-5(P<0.05, P<0.01).Conclusion:l.The cognitive dysfunction in AD can be successfully induced and simulated by injecting Aβ1-42into the hippocampal CA1region of rats. Pathological manifestations of brain tissue are the increase in Aβ deposition, hyperphosphorylation of tau protein, expression of the protein kinase CDK5and GSK-3β increased, activity was enhanced, less in the number of neurons in pyramidal cells layer of the hippocampal CA1,disordered, similar to human AD symptoms and pathological changes.2.After using HNYCF continuity gavage for12weeks, it can improve rats spatial learning and memory impairment caused by Aβ1-42. It also can inhibit the Ap deposition and tau hyperphosphorylation of hippocampal CA1region of brain tissue, improve the pathological structure of the hippocampal CA1region, improve the number of neurons of the pyramidal cells layer.3. HNYCF can improve the study learning and memory behavior of AD model caused by Aβ1-42. Its mechanism with the relevant to improve the rat brain tissue pathological structures, reduce or inhibit the formation and precipitation of Aβ, accelerate the clearance of Aβ metabolism, reduce the expression of Protein kinase CDK5and GSK-3β in the CA1 region, reduce the Tau protein hyperphosphorylation of hippocampal CA1region in brain tissue.