"liver And Spleen" As The Core Intervention High Blood Prolactin Rat Ovarian Granulosa Cell Apoptosis Mechanism

Objective:In this study, model group10and Xiaoyaosan soups and granules to the preparation of mature high prolactin hyperlipidemia rat model and appropriate dosage forms of liver and spleen core targeted intervention in high prolactin hyperlipidemia modelanimal ovarian granulosa cell apoptosis mechanism foundation. The same time, provide a new theoretical basis for the mechanism and targets of high prolactin hyperlipidemia, and proposed a new method of treatment and the prescription of safe and effective to provide a scientific basis for Chinese medicine treatment of incurable diseases of the female.Methods:Animal models of screening:ten high prolactin hyperandrogenism were randomly divided into blank control group; metoclopramide group1; metoclopramide group2; metoclopramide three groups; a group of sulpiride needle; sulpiride pin group2; diethylstilbestrolacupuncture group; the diethylstilbestrol piece group; haloperidol group; pituitary of a group; pituitary group2; In addition to the normal blank group, and were given the corresponding model. Screening again after five HPRL animal model screening. Modeling after rats were killed and blood and ovaries, by radioimmunoassay and enzyme-linked immunosorbent assay measuring serum prolactin levels and HE staining ovarian morphological changes.Modified Xiaoyaosan decoction granules:after the successful preparation of the mature animal models were randomly divided into blank group; negative control group; the modified Xiaoyaosan dose of particles group; the modified Xiaoyaosan dose decoction group; In addition to the normal blank group, and were to be treated, after the end of treatment rats were killed, blood and ovary, using radioimmunoassay and enzyme-linked immunosorbent assay measuring serum prolactin levels and HE staining of ovarian tissue morphological changes.Mechanism of ovarian granulosa cell apoptosis:in the mature animal models of successful preparation and pharmaceutical preparations were randomly divided into blank control group; negative control group; modified Xiaoyaosan high, medium and low dose group; bromocriptine group; addition to the normal blank groupwere to be treated after end of treatment, rats were sacrificed, blood and ovarian measured serum prolactin levels by radioimmunoassay and enzyme-linked immunosorbent assay using HE staining of ovarian follicle morphology changes detected by immunohistochemical methodbax and bcl-2expression differences observed ovarian granulosa cell apoptosis.Results:Ten HPRL animal model for screening and again five kinds of animal models of screening:normal blank serum prolactin, metoclopramide group1、group2、groups3serum prolactin was significantly increased (P<0.01), pituitary a groupelevated serum prolactin (P<0.05), the remaining parties module serum prolactin levels have tended to increase; compared with the follicular development in the normal blank group, metoclopramide group were the total number of modules at all levels of follicle significantlydecline significantly increased the number of atretic follicles, atretic follicles/total follicles increased significantly, the largest follicle diameter was significantly reduced.The modified Xiaoyaosan soups and granules:normal blank, modified Xiaoyaosan decoction granules were effective in promoting follicular development, reduce the role of prolactin levels; from the follicle development and lowering prolactin, the twokinds of formulations are not difference.Ovarian granulosa cell apoptosis pathway:Compared with normal blank, negative control group, prolactin levels significantly increased; the treatment groups can reduce prolactin levels, can effectively improve follicular development. Markedly enhanced the modified Xiaoyaosan enable the ovarian granulosa cells bcl-2expression, Bax expression was decreased of Bcl-2/Bax increase in the proportion of inhibition of ovarian cell apoptosis.Conclusion:Metoclopramide three groups serum prolactin levels increased significantly, significantly reduced the total number of follicle, atretic follicles increased follicle atresia/total follicles value increases, follicle maximum diameter was significantly reduced, so the optimal model; flavored Xiaoyaosan the decoctiongranules in lowering prolactin levels and follicle development, two formulations no significant difference in the final choice is simple, easy to operate decoction of the experimental formulations; modified Xiaoyaosan treatment of high prolactin hyperlipidemia has a good effect, its mechanism may beregulating ovarian granulosa cells by reducing serum prolactin levels, the effective promotion of follicular development, bcl-2, expression of bax and the ratio of this series of ways to achieve.