Skinner Much Of Microcirculation Disorder In Myocardial Ischemia-reperfusion Injury

Purpose：The experimental research is the basis of the theory of traditionalChinese medicine collaterals disease, through the blood know luo Chinesemedicine more than lust to improve rabbit myocardial ischemia and reperfusioninjury in ALT, AST, TXB2,6-keto-PGF1content and two ratio, and through thegene expression discussed more aspects of myocardial ischemia kinetekreperfusion injury myocardial microcirculation effect, and for myocardialischemia of myocardial protection offer after basic research material.Material and method：Will only50Zealand health ear white rabbitrandomly divided into5groups, and only10, respectively (1) JiaShouShu group(2) model group (3) more than50mg kinetek group A/kg (4) more than lust groupB100mg/kg (5)nitroglycerin control group， ligation rabbit leftventricular anterior descending90min,120min reperfusion, making rabbitmyocardial ischemia reperfusion of model.(1) JiaShouShu group finish all theexperiment, but not only stringing ligation rabbit coronary artery;(2) themodel group to press0.3ml/kg/min speed and dose intravenous drip saline;(3) and (4) group of according to the above to0.3ml/dose kg/min intravenousdrip more than lust;(5) to0.1ml of/kg/min speed intravenous dripnitroglycerin, continuous infusion180min stopped. After the experiment fromcarotid artery blood sampling2ml detection and the content of ALT AST; Sincethe inferior vena cava extraction3ml venous blood to detect TXB2,6-keto-PGF1content and calculator ratio; Clip about1cm3size myocardial tissue toprovide immunohistochemical and pathology examination; Will the organizationthrough the heart right ear transcriptase polymerase chain reaction (RT-PCR)methods to determine myocardial tissue cPLA2-γ gene expression level.Results：1. How A group of lust lust, more group B and nitroglycerin group compared with model group。CK and LHD content signifiantly reduce (P <0.01). Nitroglycerin group with more than lust group A comparison group B.The difference wasnotstatistically significant (P>0.05).Kinetek group B group A more and compared.The difference was not statistcally significant (P>0.05).2. The model group compared with JiaShouShu group, CK and the content of LHDsignificantly increased (P <0.01), more than lust group A, B group andnitroglycerin group group compared with model, CK and the content of LHDsignificantly reduced (P <0.01); Nitroglycerin group with more than lust groupA comparison groupB, the difference was not statistically signifcant (P>0.05);Kinetek group B group A more and compared.The difference was not statistcallysignificant(P>0.05).3. The model group compared with JiaShouShu group, TXB2,6-keto-PGF1content(P <0.01), more than lust group A, B group and nitroglycerin group group comparedwith model, TXB2,6-keto-PGF1content significantly reduced (P <0.01);Nitroglycerin group with more than lust group A comparison group B, thedifference was not statistically significant (P>0.05); Kinetek group B groupA more and compared.The difference wasnot statistically signifcant (P>0.05).4. Model group,lust and nitroglycerin group of the cPLA2mRNA expresssignificantly higher(P <0.01), more than lust lust, more than A group of groupB cPLA2-γ mRNA increased expression in normal group, but compared with modelgroup, was signifcantly lower than the model group (P <0.01); Nitroglyceringroup cPLA2-γ mRNA expression of higher than normal, but compared with modelgroup, was significantly lower than the model group(P <0.01). More than A lustlust, more group B and nitroglycerin group there were no significantdifference(P>0.05).Conclusion：1. How can signifcantly reduce kinetek little rabbit myocardial ischemia andreperfusion injury and the content of CK LHD, which can reduce the scope of themyocardial injury; Through more than lust of intervention, can reduce CK and LHD, the content of lust that can reduce more myocardial ischemia and reperfusioninjury level, and so to protect the myocardial role.2. Kinetek can significantly reduce rabbit myocardial ischemia and reperfusioninjury cPLA2-γ gene expression, suggests more than lust can restrain TXB2andPGF1ratio, and from the effective protection and myocardial, and itsmechanism may and cPLA2on gen expression.3. Two doses of the lust of myocardial protection more than the difference isnot apparent, and nitroglycerin group of control results have not obviousdifference.