| Influenza viruses are important pathogens of respiratory tract infection. Influenza pandemic produced a great menace to the people’s health in our country and even the whole world. The Advantage of Traditional Chinese Medicine anti-influenza virus is not the direct antiviral but in the overall regulation, and adjusting the immune state, improving anti influenza virus immunity. Therefore, it is profound meaning to strengthen the research of Chinese medicine anti influenza virus. This experiment discussed two kinds of different treatment between Anti-influenza formulas of shufengxuanfei and Anti-influenza formulas of jiebiaoqingli. It provided theoretical basis of clinical application for Anti-influenza formulas of shufengxuanfei、Anti-influenza formulas of jiebiaoqingli.Objective:To study the effect of the FM1influenza virus infection in mice lung index,lung injury, the content of the virus hemagglutination titer and neuraminidase in the lung tissue,and the content of IL-1, IL-8, TNF-α, MMP-1, MMP-9in lung tissue, by using the two methods ofAnti-influenza formulas of shufengxuanfei and Anti-influenza formulas of jiebiaoqingli. To provide theoretical support and reference for two kinds of traditional Chinese herbal compounding on clinical application of anti influenza. Methods:Experiment1the twoAnti-influenza formulas of shufengxuanfei and jiebiaoqingli for protection against influenza virus infection mice death.ICR male mice were randomly divided into nine groups on average, Normal control group, model control group and drug group, the breeze xuan in influenza influenza and nourish the lung qing fang)(large, medium and small dose group (hereinafter all referred to as the hydrophobic group and hydrophobic group and hydrophobic group and big group, the solution of the group, solution group).10in each group, Except the normal control group, each group under ether mild anesthesia in mice infected with influenza virus and influenza a rat lung adapt strains (FM1), with50ul41d50of flu virus droplets nasal infection,0.05ml/only, normal control group with the same amount of saline nose drops. Since infection on the day of the continuous observation for14days, mice daily observation records of deaths, and calculate the mortality and death of protection.Experiment2the two Anti-influenza formulas of shufengxuanfei and jiebiaoqingli for influenza virus infection mice lung index and the lungs pathology change.Animal groups, building, dosing method is same as above. Mice infected with influenza virus after5days, picked eye bleed to death in mice, harvesting and weighing the lung weight, lung index; Production sections under light microscopy to observe the lung tissue pathology morphological changes.Experiment3the two Anti-influenza formulas of shufengxuanfei and jiebiaoqingli for influenza virus infection mice blood clot drop degree and the influence of neuraminidase activity.Animal groups, building, dosing method is same as above. Respectively take5days after infection of lung tissue in mice, put in homogenate machine, according to the lung weight (g)/saline solution (ml)=1/9of the proportion in physiological saline, grinding into lung homogenate, centrifugal (2000r,10min,4℃), and then take the supernatant fluid according to the conventional method trace blood clotting experiment and detection of virus neuraminidase content.Experiment4the two Anti-influenza formulas of shufengxuanfei and jiebiaoqingli for influenza virus infection mice lung tissue, IL-8, TNF, IL-1-alpha, MMP1, the influence of the content of MMP-9.Animal groups, building, dosing method is same as above. Respectively take5days after infection of lung tissue in mice, put in homogenate machine, according to the lung weight (g)/saline solution (ml)=1/9of the proportion in physiological saline, grinding into lung homogenate, centrifugal (2000r,10min,4℃), and then extract the supernatant fluid, clip art)(double antibody by ELISA method to detect, IL-8, TNF, IL-1-alpha, MMP1and the expression level of MMP-9.Results: Experiment1the two Anti-influenza formulas of shufengxuanfei and jiebiaoqingli for protection against influenza virus infection mice death.A mortality rate of80%, according to the results of model group (survival rate was20%), the positive control drug tamiflu mortality was10%, by the chi-square test, P<0.01, death has obvious protective effect; Hydrophobic groups in the mortality rate of18.2%, compared with model group, the chi-square test, P<0.01, also has the obvious death protection; Hydrophobic group reconciliation group of death rate was27.3%, compared with model group P<0.05, suggesting that the death of also has a certain protective effect; In the other groups were no significant difference compared with model group. In addition, the drug group and hydrophobic group, P>0.05, no difference between them, that tamiflu group and hydrophobic group of death protection.Experiment2the two Anti-influenza formulas of shufengxuanfei and jiebiaoqingli for influenza virus infection mice lung index and the lungs pathology change. Mice infected with influenza virus FM1, model of lung index is significantly higher than normal group (P<0.01). Compared with model group, drug group and the two kinds of anti-flu medicine group after treatment for lung index of mice has lower effect, showed obvious inhibitory effect on lung index. Among them, the lung index of hydrophobic group, the solutions of the big group compared with model group (P<0.05); Tamiflu, hydrophobic groups, hydrophobic groups, solution in the group, the group of lung index compared with model group (P<0.01). Hydrophobic groups in the lung index, inhibition rate was33.33%, the effect is obvious, but in the two kinds of prescription for the inhibition of lung index than tamiflu group (lung index, inhibition rate of43.86%) effect is good. HE observed dyeing: normal group, the lung tissue of mice structure form in good condition, alveolar wall thickness and size are normal, not seen around the inflammatory cell infiltration. The lung tissue of mice model with inflammatory cells and normal lung tissue structure of the form will disappear, thickening of alveolar walls, a large number of lymphocyte, neutrophil infiltration, congestion can be seen in the blood vessels. Tamiflu group restore alveolar sacs and alveolar tissue structural forms, and I see a small amount of inflammatory cells infiltration. Chinese medicine groups each have different degrees of improvement the inflammatory pathological changes of lung tissue, the therapeutic effect of hydrophobic group was obviously, reduce inflammatory cells, the morphological structure of lung tissue basically recovered.Experiment3the two Anti-influenza formulas of shufengxuanfei and jiebiaoqingli against influenza virus for influenza virus infection mice blood clot drop degree and the influence of neuraminidase activity.No virus in lung in normal mice, the lowest content of neuraminidase. Mice after infected with influenza virus FM1, model group of virus blood clotting sharply drops degree and content of neuraminidase, compared with normal group have significant difference, P<0.01). Other groups compared with model group P<0.01, significant difference. After antiviral treatment, drug group and the traditional Chinese medicine group of virus drops degree of coagulation and neuraminidase levels were significantly reduced, played a very good antiviral effect. The treatment group compared with model group, P<0.01). Tamiflu is one of the groups of treatment effect is best, in the hydrophobic group.Experiment4the two Anti-influenza formulas of shufengxuanfei and jiebiaoqingli for influenza virus infection mice lung tissue, IL-8, TNF, IL-1-alpha, MMP1, the influence of the content of MMP-9.Mice after infected with influenza virus FM1, in lung tissue homogenate of mice model, IL-8, TNF, IL-1-alpha levels increased significantly, compared with normal group significant difference, P<0.01); Compared with model group, drug group and hydrophobic group and hydrophobic group, hydrophobic groups, large groups, P<0.01solution in group, model group compared with solution team, P<0.05were significantly decreased in the lung tissue, IL-8, TNF, IL-1-alpha levels, have obvious inhibitory effect. MMP1in model group rats lung tissue, MMP-9levels increased significantly compared with normal group, P <0.01); Compared with model group, drug group and hydrophobic group and hydrophobic group and big group, the solution of the group, group of MMP1had different degrees of lower levels, P<0.01); And hydrophobic groups, no significant change. Compared with model group, drug groups, hydrophobic groups, solution in the group, the group significantly lower levels of MMP-9, P<0.01); Hydrophobic group also has decreased, P<0.05; Group and hydrophobic group settlement, big change has no statistical significance.Conclusion:The above two formulas for the treatment of infectious influenza virus pneumonia mice effect has obvious difference, The shufengxuanfei is superior to the general effect of the jiebiaoqingli, with shufengxuanfei formula of honeysuckle, forsythia, folium isatidis, radix isatidis, etc have obvious antiviral and antipyretic anti-inflammatory effects. Is also working with mice after infected with influenza virus both on table, hot wind poison attack to the pathogenesis of lung, and exogenous cold origin, in the hot, indicates the propaganda in the pathogenesis of lung, pulmonary xuan drop loss, more in line with shufengxuanfei influenza pathogenesis. Therapeutic dose to the dose effect is the best. |
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