| From2003,"Shenzhou V" manned space flight, to2012"divine nine" and "Temple No.1," the manual rendezvous and docking success, the Chinese people to achieve their dream of flying, and toward the long-term space exploration strides. However, a series of weightlessness on the human body physiological and pathological problems, especially in muscle atrophy is very serious, it not only affects the astronauts perform space missions of security, but also restricts the astronauts return to the ground again after the adaptability become an urgent Space Medicine problem.Currently muscular atrophy for weightlessness countermeasures taken mainly include chemical drugs and physical therapy, these methods are expensive, complex, and take up a lot of astronauts working time. Chinese traditional medicine is a major feature of medical, aerospace medicine TCM theory to solve the problem equally applicable. At present, a large number of studies have shown that weight loss medicine muscle atrophy on the effect of interference, but its mechanism remains unclear.This topic intends to skeletal muscle atrophy weightlessness for TCM analysis, the application of the appropriate prescription, expand the experimental studies to disease measuring machine, measured in square card, try to ask weightlessness induced muscle atrophy syndromes characteristics and intervention rules; and from the medical point of trying to explain the molecular mechanism of trad it ional Chinese medicine, preliminary exploration, in the field of medicine in the development of China’s manned space preliminary exploration.The subject intends to on TCM Pathogenesis weight loss of skeletal muscle atrophy, the application of the appropriate prescription, expand the experimental study, disease measuring machine, measured in square card, try to make weight loss of skeletal muscle atrophy TCM syndromes and intervention law; from the point of view of molecular medicine to try to explain Chinese medicine on preliminary exploration, preliminary exploration for the development of Chinese medicine in China’s manned space field.Theoretical discussionWeightlessness muscular atrophy belong disuse muscle atrophy, according to the pathological changes and symptoms described, grouped into Chinese "flaccidity" category. Present for the disease pathogenesis and dialectical theory of governance did not reach consensus, is still in the Preliminary stage.Astronauts into space, earth (ground) gas sudden decline, involved in the spleen, the spleen is not healthy, deficiency of blood stagnation, thereby causing atrophy. Therefore considers temper wasting, blood loss and muscle atrophy is weightlessness main pathogenesis.Governance when the spleen wing muscles, qi and blood.Chosen to blood circulation as the main rule is the BYHWD and to qi and blood as the main rule is to subtract Buzhongyiqitang intervention.Experimental studyThe main part of the experiment comparing designs based on different dialectical thinking two experiments simulated weightlessness Prescription for two weeks against the effects of muscle atrophy.Part I:BYHWD and subtraction Buzhongyiqitang weightlessness muscular atrophy on the interventionExperiment by comparing each group of mice soleus and gastrocnemius muscle index, found in mice caused by tail suspension soleus and gastrocnemius muscle index fell, BYHWD right mouse tail suspension no significant muscle atrophy in rats.Second experiment showed that, compared with BYHWD, subtraction Buzhongyiqitang mouse tail suspension significantly increased gastrocnemius muscle index and fiber cross-sectional area of the weightlessness has some muscle atrophy in rats.The effects of different doses of experimental three subtraction Buzhongyiqitang weightlessness muscular atrophy on the intervention effect was found in the dose subtract ion Buzhongyiqi tang (equivalent to2times the clinical human dosage) on weight loss more muscle atrophy in rats obvious.In summary, BYHWD right weightlessness muscular atrophy without significant intervention effect, addition and subtraction can significantly improve Buzhongyiqitang mouse tail suspension index and antigravity muscle fiber cross-sectional area. In Huoxuetongluo as the main rule is the BYHWD right weightlessness muscular atrophy without significant role in Spleen Qi and blood wing muscles for the rule is the subtraction Buzhongyiqitang right weightlessness significant muscle atrophy rats. Prove the key to weight loss muscle atrophy machine that temper wasting disease, rather than stasis and qi stagnat ion.Part Ⅱ:Modified Buzhongyiqitang mechanism of actionExperiment one:in vitro cell culture methods to prove subtraction Buzhongyiqitang promote C2C12myoblast proliferation and differentiation, promote cell Myogenin, Myhc expression; validation in disease models, plus or minus Buzhongyiqitang improve Tail hanging mouse muscle differentiation and regeneration. C2C12oxidative metabolism in vitro demonstrate improved functionality.Experiment Ⅱ:According to the taste of herbs and subtraction Buzhongyiqitang the main active ingredient of the chemical structure, the use of bioinformatics, network pharmacology methods to predict the potential role of addition and subtraction Buzhongyiqitang targets screened out and Modified Buzhongyiqitang of the targets:muscle growth and oxidative metabolism related molecules NCoR1.Verify predictions, subtraction Buzhongyiqitang and tail hanging down C2C12mouse NCoRl express and promote their growth and oxidative metabolism and muscle-related target gene expression.In summary, the key to weight loss, muscular atrophy pathogenesis that temper wasting, transport and not healthy, Qi stagnation blood, flesh loss of Ru Yang. With Spleen Qi, and blood wing muscles for the rule is the subtraction Buzhongyiqitang muscle atrophy on weightlessness obvious intervention effect, and the dosage to2times the human clinical dose, intervention works best.NCoRl mediated oxidative metabolism and muscle growth and associated signaling pathways. Modified Buzhongyiqitang myoblasts NCoRl by inhibiting the expression promotes cell proliferation, differentiation and oxidation function, thereby inhibiting muscle NCoRl mouse tail suspension expression, thus contributing to the muscle regeneration capacity, improve muscle oxidative metabolism, against the weightlessness muscular atrophy. This could be plus or minus Buzhongyiqitang intervention weightlessness muscular atrophy is an important way. |
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