The Quality Of Tibetan Medicine Himalayan Mirabilis Jalapa Evaluation Research

Radix Mirabilis Himalaicae is the dry root of Mirabilis himalaica (Edegw) Heim,its Tibetan transliteration is Bazhu.As a commonly used Tibetan medicine,this drug can drying huangshui,nourishing kidney, diuresis and expelling stone.In2006,the artificial cultivation base of Radix Mirabilis Himalaicae has been based in Gongga by Tibet Tibetan medicine college.This makes Radix Mirabilis Himalaicae become the first cultivated Tibetan medicine materials.However,there is no a quality standard can evaluate the quality of Tibetan medicine scientifically,the problem that whether there is any differences in quality between authentic and not authentic medicinal herbs or wild and cultivated medicinal materials is failed to solve either.In this experiment,we collected8Radix Mirabilis Himalaicae samples,including the wild medicinal materials collected from Tibet and Gansu,and the culture ones collected from Tibet.By observing and describing their macro-and micro-structure,we determined the macroscopical and microscopic characteristics.By carrying out a series of chemical experiments, the type of the chemical composition of the herbs was determined roughly.TLC of3polarity parts was established.Set up the extraction and determination methods of the medicinal materials’total saponins,total polysaccharides and total flavonoids, and determined the components’content of the8samples. The followings are details.1. Macroscopical identification characteristics of Radix Mirabilis HimalaicaeRadix Mirabilis Himalaicae herb pieces consists of transverse sections,longitudinal sections and oblique sections.Transverse sections,circular or elliptical,its diameter and thickness is2-4cm and0.3-0.7cm, respectively;Oblique sections are oval,which diameter is0.3-1cm. Rind (yellow-brown,rough),texture (hard).There is concentric annular layer and visible scattered vascular bundles in the gray section; The longitudinal section has vertical lines.Displaying mealiness. Smelling weak, tasting slightly acrid and having a sense of barbed hose when chews it.2.Microscopic characteristics of Radix Mirabilis HimalaicaeSelecting the medicinal materials of Radix Mirabilis Himalaicae to make paraflfinic blade of transverse section and powder blade.Observing its features:outboard series cell wall of cork layer in root section suberization, inside eight to ten layer cell wall suberization is not obvious.Ectophloic type of the tertiary vascular bundles arrange indirectly a ring.Xylem conduit arrange radially as single or groups.Parenchyma cells contain starch granule and sometime contain oxalic acid calcium needle crystal beams.The powder’s color is gray.Starch granule is numerous, single grain is round or oval, its diameter is4-10μm, compound grains is constituted by2-eight single grain.Oxalic acid calcium needle crystal is numerous, bobbed or scattered, length is12-130μm.Catheter is scalariform vessel or reticulate vessel, the diameter is25-35μm.3. Chemistry chromogenic experiments of Radix Mirabilis HimalaicaeA variety of chromogenic reaction results suggest that, the Himalayan mirabilis jalapa’s types of main chemical components including saponins, flavonoids, polysaccharide, tannic acid and small amounts of amino acids.4.TLC identification of Radix Mirabilis HimalaicaeIn this experiment,we established three different TLC chromatography methods for different polarity extract of the herb,which can separate the chemicals in the Radix Mirabilis Himalaicae’s extract effectively, the spots on TLC were clear, the Rf value is reasonable.5.The method of extraction and content determination for the chemical component of Radix Mirabilis HimalaicaeThe methods that determined the content of total saponins, total polysaccharide and total flavonoids by UV spectrophotometry were established.Through the methodology,the methods above were qualified in linearity, repeatability, precision, stability, sample recovery rate,can be used as the content determination methods of total saponins, total polysaccharide and total flavonoids in Radix Mirabilis HimalaicaeThrough single factor and orthogonal test,we optimize the extracting conditions of total saponins,total polysaccharides and total flavonoid,the methods above are convenient, scientific and stability, and with the high yield of composition, can be used as a laboratory prepared methods of total saponins, total polysaccharide and total flavonoids in Radix Mirabilis Himalaicae.6.Comparison of chemical composition content between different samples of Radix Mirabilis Himalaicae.Content of total saponins, total polysaccharides and flavonoids in Eight samples is0.473%～0.473%,5.039%～13.601%and1.470%～3.717%respectively. The chemical composition content of different samples have differences.Grouping the8samples into2group in accordance with their origin and compare the content between them.The analysis results show that the content of total saponins, total polysaccharides and flavonoids in the sample which produced in Tibet are higher than the ones which produced in Gansu.Grouping the8samples into2group in accordance with their way of growth,we found that there were no significant differences between the wild samples and the cultivated ones in the content of total saponins and flavonoids,but in the content of total polysaccharide, the cultivated products have the higher content than wild ones.7.Comparison for the quality of authentic and not authentic medicinal materials、wild and cultivated medicinal materialsBy compare the quality of medicinal materials from different regions, we demonstrate that Tibetan drug’s quality is better than Gansu ones.By compare the quality between the wild medicinal materials and the cultivated one, we demonstrate that the two have no obvious difference.Cultivating medicinal materials have the possibility to instead of the wild one.