Low Power Laser On One-time Centrifugal Exhaustion Movement Rats Microdamage Repair Function Of Achilles Tendon

Objecties: this study uses low power semiconductor lasers, does thecomprehensive study of the one-time centrifugal exhaustion movement rats’ changes ofthe protein content after platform running movement about their early achilles collagentypeⅠ, collagen typeⅢ, IGF-1, combined with the achilles tendon organization and theultrastructure observation, from different levels and angles such as organizationmorphology, biochemistry and molecular biology and so on, to discuss the roles of andmechanism of the low power laser radiation on achilles tendon repair process afterexhaustion sports, to explore the application methods of low power laser in sportsmedicine field.Methods: this experiment used the exhaustion sets pattern and low power laserirradiation two interventions:60male8weeks of age Wistar rats, randomly divided into:blank control group (n=6) and exhaustion running model making group (n=54), andthe later group also divided into:①model making control group (n=6)②the naturalhealing group (n=24): one-day group, two-day group, three-day group, seven-daygroup.③laser treatment group (n=24): after building model one-day treatment group,two-day treatment group,3-day treatment group, seven-day treatment group. Takinglaser irradiation daily after the completion of the model building of exhaustion run. Thetest methods and index:1. HE dyeing observation of the structure changes ofrats’collagen fibers achilles.2. Testing the content of achilles tendon hydroxyprolinewith samples buck solution.3. With Double antibody sandwich ABC-ELISA methodto test the collagen type Ⅰ, collagen type Ⅲ and IGF-Ⅰ protein content.Results:1. Observation through HE dyeing light and transmission electronmicroscope show that low power laser has positive roles for repairing the microdamageof Achilles tendon and improving tendon cells’secrete function.2.One-time centrifugalexhaustion movement rat model control group Achilles tendon made hydroxyproline, collagen typeⅠ, collagentype Ⅲcontent and blank compared with controls aresignificant difference (P <0.05), and the results show that made model success3.Hydroxyproline content changes: for laser treatment, there is no difference betweenone-day, two-day groups and the natural healing1one-day, two-day groups; lasertreatment of3-day grop has significantly higher healing rarethan the natural healing3-day group (P <0.05),7-day of laser treatment group has significantly lower rate thanthe natural healing7-day group (P <0.05).4. The IFG-Ⅰ content changes: lasertreatment one-day, two-day, three-day groups have higher content of IFG-I than those ofthe relevant natural healing groups (P <0.05); Laser therapy seven-day group hassignificantly lower IFG-I than the natural healing seven-day group (P <0.05).5.Collagen content changes: laser treatment group collagen type Ⅰcontent changes arebasically same as the natural healing group in changing trend, but the laser treatment3-day,7-day groups have higher collagen type Ⅰ content than the relevant naturalhealing groups (P <0.05). For laser treatment group, collagen type Ⅲreach the peak in2days, significantly higher than the natural healing groups(P <0.05), laser treatment3-day,7-day group are significantly lower than the natural healing3-day,7-day groups(P <0.05). Laser therapy group7-day group has similar collagen type Ⅲcontentcompared withblank control group.Conclusion: Low power laser radiations have stimulative effect one-timecentrifugal exhaustion movement rats microdamage repair of Achilles tendon. Lowpower laser irradiation can make one-time centrifugal exhaustion movement rats speedup Achilles tendon collagen metabolism, adjust the extracellular matrix, reduce scarappear, improve the quality of the Achilles tendon microdamage repair.