Caveolin-1Expression In The Brain Of Mice And Epileptic Rats And Learning And Memory

Caveolin-1(Cav-1),a marker protein of Caveolae,plays an important role in cholesterol transport,membrane assembly,signal transduction and tumorigenesis.Cav-1is related to the physiological and pathological changes of brain function and affects neural development,synaptic plasticity,the occurrence of neurodegenerative disease,and so on.Previous work in our laboratory showed that expression of Cav-1is closely related to learning and memory, the mechanism of which maybe related to activation of the Cav-1-MAPK-CREB signaling pathway,suggesting that Cav-1may be involved in plasticity of the central nervous system.The purpose of this study was to investigate the relationship between animal learning and memory and expression and distribution of Cav-1protein in brain in normal and pathological conditions,providing the experimental basis to further elucidate the role of Cav-1in learning and memory.The open field test is used to detect spontaneous locomotor activity of mice.The Y-maze bright-dark discrimination learning model was used to determine the learning scores of mice. The expression of Cav-1protein in cortex and hippocampus of the mice was detected by Western blotThe correlation analysis was used to understand the relationship between learning scores and the expression of Cav-1protein in different parts of brain.Epileptic rats were generated by lithium-pilocarpine-induced status epileptogenesis.Immunohistochemistry was used to analyze the expression of Cav-1protein in cortex and hippocampus of the normal rats and rats at day4,day30after status epilepticus.The expression of Cav-1protein in the hippocampus of rats was detected by Western blot at day l,day4,day7,day15after status epilepticus.The Morris water maze test was used to detect the capacity of rat on learning and memory from the25th day to the30th day after seizures.The results showed that (1) the learning scores have distinct correlation with the expression of Cav-1in the cortex of mice.In open-field test, there were no significant differences in the number of crossing and rearing of excellent group and bad group. Cav-1protein expression of excellent group in the cortex was significantly higher than that of the bad group (P<0.01). While there were no significant differences in the two groups in the expression of Cav-1protein in hippocampus.There were no significant differences in the brain weight/body weight of excellent group and bad group.(2) Induction of epilepsy could increase expression of Cav-1in hippocampus of rats,which was mainly detected in the area of CA1,CA3, and the dentate gyrus(DG) of rats at day4after status epilepticus comparing with the normal rats.The expression of Cav-1in hippocampus of rats at day l,day4,day7after status epilepticus was higher than normal rats.(3) Spatial learning and memory between normal rats and epileptic rats had no significant differences24days after Lithium-Pilocarpine-induced acute epileptic seizures.In place navigation test,at the same time point,the percentage of swimming time in the target quadrant,the escape latency and swimming distance of the normal rats compared with the epileptic rats,had no significant differences.In spatial probe test,the number of cross-platform of the normal rats compared with the epileptic rats,had no significant differences yet.Conclusion:(1) The learning scores had distinct correlation with the expression of Cav-1in the cortex of mice.(2) Induction of epilepsy could increase expression of Cav-1in hippocampus of rats.(3) Spatial learning and memory between normal rats and epileptic rats had no significant differences24days after Lithium-Pilocarpine-induced acute epileptic seizures..