The Effect And Mechanism Of Traditional Korean Medicine Sanshen Powder On Learning And Memory In Vascular Dementia Rats

[Object]:This study was to investigate the preliminary mechanism of traditional korean medicine sanshen powder on the function of learning and memory in Vascular Dementia (VaD) rats, which contributes to supply theoretical basis for clinical experiments.[Method]The acute toxicity test of traditional korean medicine sanshen powder: Maximum delivery was used. Wistar rats were used weight among220±30g.20male and female rats were selected and then random divided into four groups (Radix Polygalae group, Drgonsbones group, Acorus gramineus group and control group),10rats in each group. They were intragastric administrated three times a day and the does were used according to Radix Polygalae groups for36g/kg, Drgonsbones groups for240g/kg, Acorus gramineus groups for42g/kg and control groups for10ml/kg distilled water instead. Rats were alive to be observed for sequent7days after administrated for one day. And then they were sacrificed by dislocation and were dissected to check each organ with the naked eyes and determine the security of these drugs.The effects of traditional korean medicine sanshen powder on learning and memory in VaD model of rats:42male rats were random divided into6groups, sham-operation group, models group, hydergine group, sanshen powder high does group, sanshen powder middle does group and sanshen powder low does group,7rats in each group. Vascular dementia models were induced by Repeated clipping bilateral common carotid artery after Intraperitoneal Injection of Sodium Nitroprusside (2.5mg/kg). after the creation of the model, the rats were injected corresponding drugs for4weeks, and then process Morris water maze to test the ability of learning and memory of rats, while determining the glutathiose content in serum by biochemistry mechod.[Results]1.Acute toxicity test:①General state:there’s no death observed in any of Drgonsbones, RadixPolygalae, Acorus gramineus or control groups. There were no abnormal performances about fur, feeding, drinking or movements. All were in good condition.②The Maximum delivery does:The maxium daily does in Drgonsbones, Radix Polygalae, Acorus gramineus groups were240g/Kg、36g/Kg、42g/Kg, which were400,300,233times more than adults.③Anatomical observation with the naked eye:there were no significant changes in any organ of heart, liver, spleen, lung, kidney, gastrointestinal tract which dissected from experimented rats observed with the naked eye, which indicate that Drgonsbones, Radix Polygalae, Acorus gramineus do not induce Pathological injury of parenchymal organs.2.The effects of traditional korean medicine sanshen powder on learning and memory in VaD model of rats①The effects of traditional korean medicine sanshen powder on general state and weight:during the experiments, the general state were well in all four groups. Moreover, there were no significant changes in weight among these four groups in fourth weeks (P>0.05).②The effects of traditional korean medicine sanshen powder on spatial memory and Space exploration:the platform latency found by rats was significantly shortened ((P<0.05))in sanshen powder high does group, compared with control group. The through platform numbers were significantly increased in sanshen powder middle does and sanshen powder low does and control groups (P<0.01, P<0.05).③The effects of traditional korean medicine sanshen powder on GSH in ser um:The content of GSH in rat serum in sanshen powder high, middle and low does groups were all higher than model group (P<0.01)[conclusions]1. Drgonsbones, Radix Polygalae, Acorus gramineus which are the components of traditional korean medicine sanshen powder were low deleterious.2.traditional korean medicine sanshen powder improve the ability of learning and memory in VaD model rats may through increasing the GSH activity in serum.