| Microbes play an important ecological role in the nature, before application of the abundant microbial resources, microbial classification is needed. Microbial taxonomy as an evolving subject provides a lot of research achievements of microorganisms, and increasingly become an accurate catalog and information about abundance microbes on earth. Our Country has wide microbial resources, but researches of the isolation and identification of microorganisms are still limited.The genus Flavobacterium (family Flavobacteriaceae, order Flavobacteriales, class Flavobacteriia, phylum Bacteroidetes) was first proposed by Bergey et al.(1923) and an extensively emended description of the genus was given by Bernardet et al.(1996). The genus Flavobacterium currently consisting of over60species, and16species were isolated from China. The genus accommodates Gram-staining-negative, aerobic, rod-shaped, non-spore-forming, yellow-pigmented bacteria that have menaquinone-6as the major respiratory quinone and are non-motile or motile by gliding. Flavobacterium strains have a DNA G+C content range of30-41mol%and most Flavobacterium strains contain iso-Cis:o as the major fatty acid.A Gram-staining-negative, rod-shaped, strictly aerobic, yellow-pigmented bacterium, designated strain DK69T, was isolated from soil collected from the waste liquid treatment facility of Bafeng Pharmaceutical Co., LTD in the city of Enshi, Hubei province, China. Phylogenetic analysis based on16S rRNA gene sequences placed strain DK69T within the genus of Flavobacterium in the family Flavobacteriaceae. Highest sequence similarities were found with Flavobacterium cauense (96.9%), Flavobacterium saliperosum (96.3%) and Flavobacterium suncheonense (95.7%). Strain DK69T grew at4-32℃(optimum,28℃), at pH6.0-8.0(optimum, pH7.0) and with0%NaCl. The major fatty acids (≥5%) were iso-C15:0, iso-C17:1ω9c, C15:0, iso-C17:03-OH, and iso-C15:03-OH, the major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid, and the major respiratory quinone was menaquinone-6. The genomic DNA G+C content was34.4mol%. Polyphasic taxonomic studies showed that strain DK69T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium enshiense sp. nov. is proposed. The type strain is DK69T (=CCTCC AB2011144T=KCTC23775T).A strictly aerobic, Gram-staining-negative, rod-shaped, non-motile, yellow-pigmented bacterial strain designated BX12T, was isolated from soil collected from the peak area of Wudang Mountain in the city of Shiyan, Hubei province, China. Phylogenetic analysis of the16S rRNA gene sequences showed that strain BX12T was most closely related to Flavobacterium subsaxonicum WB4.1-42T (95.9%), followed by Flavobacterium beibuense F44-8T(95.6%) and Flavobacterium rivuli WB3.3-2T(94.1%). Strain BX12T grew at4-32℃(optimum,28℃), at pH6.0-8.0(optimum, pH7.0) and with0-2%NaCl (optimum,0%).The major fatty acids (≥5%) were summed feature3(comprising C16:1ω7c and/or C16:1ω6c), iso-C15:0, C16:0, iso-C17:03-OH, and C16:03-OH. The major polar lipid was phosphatidylethanolamine, and the major respiratory quinone was menaquinone-6. The genomic DNA G+C content was43.9mol%. On the basis of a high number of phenotypic differentiating properties and phylogenetic uniqueness, Flavobacterium hauense sp. nov. is proposed, with type strain BX12T (=CCTCC AB2012197T=KCTC32147T).In this study, we performed polyphasic taxomony analysis of two strains DK69and BX12isolated from different soil samples. We analyzed the type strains of related Flavobacterium species and emended the genus description. This study identified novel bacterial species and enriched the knowledge of microbial diversity. |
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