Study On Potential Toxic Mechanism And Evaluation Of Toxcity Of Anthraquinone Pigments Through The Resonance Light Scattering

DNA intercalator theory is introduced to study the mechanism of potential toxicityof anthraquinone pigments and to evaluate their toxicities quickly in the paper. Thesaturation values binding with DNA of alizarin, purpurin, and1-hydroxyanthraquioneare calculated through the resonance light scattering results and compared with ethidiumbromide, adriamycin and mitoxantrone’s saturation values binding with DNA with thepurpose of evaluating toxicity of alizarin, purpurin,1-hydroxyanthraquione quickly andbuilding a rapid warning method of toxicity of anthraquinone pigments.The saturation values binding with DNA of alizarin, purpurin, and1-hydroxyanthraquinone are0.20,6.41,0.18respectively, while the ethidium bromide,adriamycin, mitoxantrone’s saturation values binding with DNA are14.90,10.61,3.41respectively, which are calculated through the resonance light scattering spectra. Theabove results infer that the toxicity of purpurin is between those of adriamycin andmitoxantrone, and the toxicities of alizarin and1-hydroxyanthraquinone are smallerthan that of mitoxantrone. Meanwhile, the toxicities of three are much smaller than thatof ethidium bromide.Factors affecting saturation values binding with DNA of alizarin, purpurin, and1-hydroxyanthraquione are also studied through the resonance light scattering spectra inthis paper. Histamine, glucose, acid and alkali could decrease the alizarin’s saturationvalue binding with DNA whereas aspartic acid, tryptophan, NaCl could increase thealizarin’s saturation value. Glucose, leonine, VB12, BSA could decrease the purpurin’ssaturation value binding with DNA whereas CaCl2could increase the purpurin’ssaturation value binding with DNA. Folic acid, histidine, threonine and alkali coulddecrease the1-hydroxyanthraquinone’s saturation value binding with DNA whereasNaCl, glucose, BSA and acid could increase the1-hydroxyanthraquinone’s saturationvalue binding with DNA. So the toxicities of alizarin, purpurin and1-hydroxyanthraquione could be increased or reduced by changing environments fromthe above results.The interaction relationship between alizarin, purpurin,1-hydroxyanthraquinoneand DNA were studied through fluorospectrophotometry, UV spectrophotometry andagarose gel electrophoresis with ethidium bromide as the reference. Three pigmentscould react with DNA as ethidium bromide, and the order from strong to weak isethidium bromide, purpurin, alizarin, and1-hydroxyanthraquione. Ability to inhibitbacterial growth of alizarin, purpurin, and1-hydroxyanthraquione were also studiedthrough bacteriostatic test and the sequence from strong to week is ethidium bromide,purpurin, alizarin, and1-hydroxyanthraquione. The above results are in accord with theresults of the method in the paper, therefore, the method in the paper is feasible andreliable.The interaction between three anthraquinone pigments and E.coli were studied through the resonance light scattering spectra and compared with ethidium bromide,adriamycin and mitoxantrone. Alizarin, purpurin,1-hydroxyanthraquione could interactwith genome DNA of E.coli and could exist in living cells as well as ethidium bromide,adriamycin and mitoxantrone. The strongest interaction time of compounds could beobtained by the method of this paper. The strongest interaction time of ethidiumbromide, adriamycin, mitoxantrone, alizarin, purpurin and1-hydroxyanthraquione are4.0h,5.0h,3.5h,6.5h,4.5h, and4.0h respectively.