One–step Emulsion Electrospun Bimodal Scaffold For Cell Growth

21st century is the electrospinning’s century. Electrospinning technology has greatpotential and developing by leaps and bounds. Improved scaffolds fabricated byelectrospinning with good biocompatibility and good mechanical properties mimic thephysical and chemical structure of extracellular matrix (ECM). Supporting suitablestructure to growth factors keeping integrity and vitality, scaffolds are widely used intissue engineering especially in soft tissue repairing.Extracellular matrixes has a three-dimensional (3D) structure possess both micro-and nano-elements. Mimic the nanostructure of ECM, Traditional electrospinningscaffolds only compose of nanofibers are beneficial to cell adhesion and cellproliferation. Small pore due to the small diameters results to the disadvantage of cellinfiltration.Electrospinning prepared scaffolds range from nano-to micro-fiber. Theelectrospinning has experienced from a single component to the mixed equipment, alsocompleted the breakthrough from the two-dimensional structure to thethree-dimensional structure. Each link in electrospinning process can be controlled,including the internal solution factors (surface tension, solution properties andconductivity, etc.) and external factors (spinning distance, voltage, feeding speed, etc.).Over the past few decades, lots of study focus on fine regulation of electrospinningprocess. To improve the syringe or receiver, decoration on the surface of scaffolds topromote the cell behavior of adhesion, migration and proliferation. But traditionalnanofibers with small pore due to its small size still limits the cell infiltration.In order to overcome the problem, our study introduce micro-scale structure toengage cell infiltration. The most important thing in this study is that scaffolds areelectrospun in a simple one-step process. Using a standard single syringe electrospinsetup hold great potential in large-scale industrial manufacturing.①The preparation of bimodal fiber scaffoldPCL nanofiber and bimodal scaffolds fabricated by electrosping with HFIP andDCM as solvent. introduced emulsification in the electrostatic spinning process,regulate the fiber diameter by changing composition of the solution. Using lightmicroscope to observe the droplet distribution. Using scanning electron microscope (SEM), transmission electron microscopy(TEM) and Fourier infrared spectroscopy (FTIR), mechanical test to characterizephysicochemical properties of scaffold.②The interaction between cells and scaffold materialsCulture human immortalized the epidermal cells (HaCat) on PDH02, PDH13,PDH11, PDH31and PDH20five kinds of scaffolds, SEM and MTS was used todetected cell adhesion on the material, found that five kinds of materials have goodbiocompatibility.③Cell infiltrationAn in vitro Transwell was used to determine the response to cell infiltration intofive different scaffolds. The results showed that nanofiber gots very good adhesion butweak cells infiltrating ability; combined micro-with nanofiber support the cell adhesionand also promote cell proliferation and infiltration within the scaffolds.Topic of our research: changing PCL solution component by emulsification beforeelectrospinning. We use one-step elmusion electrospinning to prepare PCL/HFIP/DCMbimodal scaffold to mimic the ECM structure. Different scaffolds have goodbiocompatibility and mechanical properties for cell adhesion and proliferation. Theresults provide a theoretical basis for large-scale industrial.