Study On The Natural Plant Colorant Extracting Process By Biological Enzyme

In recent years, with the increasing of environmental awareness of people, the pollutionproblems which are caused by the synthetic dye production and application processes havebeen attracted people’s attention. Under the wave of the green revolution, along with thedyeing and finishing industry develop toward to ecological, energy-saving and emissionreduction, natural dyes are taken into consideration. Most of natural dyes are Chinese herbs,which have good medical value and benefit health. It is the tendency to develop and use thenatural dyestuff.This article has carried on the discussion to extract the natural dyestuff process by theenzyme. In order to enhance the natural dyestuff’s extraction rate, in the experiment mainlyused the biological enzyme. The basic mechanism of using the enzyme method to extract thenatural dyestuff is that was acted on the surface layer material, and then caused the cell wallsand the intercellular substance structure changed loosen, expansion, collapse and so on. Thusthe area that the effective component was extracted was changed larger, and the transferresistance was reduced, so the rate of extraction was enhanced. The phellodenron, the radixlithospermi, the gingko leaf were selected to extracted the dyestuff. According to thedifferent spot, the sole enzyme, the complex enzyme, and adding complex enzyme step bystep separately to carry on the extraction processing. The single factors were done to disscussthe effect of the different factors on the extract rate of the dyestuff, and the optimizedprocessing was definited, simultaneously the dyeing processes were definited by dying thecotton and the woolen fabric.The experimental result indicated that the extraction ratio by the method of adding theenzyme was significantly higher than that of traditional water extraction. The phellodenronused the sole enzyme extraction, compared with the water extraction, the extinction rate wasenhanced27%; The radix lithospermi used the cellulase and the xylanase conplex enzymeextraction, compared with the water extraction, the extinction was enhanced2.5times; Thegingko leaf selection pectolase and the cellulase affect successively, the extinction wasenhanced30%, compared to the water extraction; And the biological enzyme has not affected to the extraction natural dyestuff’s structure. The phellodenron dyestuff optimal extractionprocess is that the liquor ratio=1:10, the zymohydrolysis time is80min, the extractiontemperature is80℃,the extraction time is90min,Mcellulase:Mphellodenron=1:110; The radixlithospermi dyestuff optimal extraction process is that the liquor ratio=1:15, thezymohydrolysis time is40min, the extraction temperature is50℃, the extraction time is60min, Mcellulase：Mxylanase=3：2, Mcellulase：Mthe radix lithospermi=1：100;The the gingko leafdyestuff optimal extraction process is that the liquor ratio=1:10, Menzyme：Mthe gingko leaf=1：100,Mpectinase：Mcellulase=1：2, the zymohydrolysis time is60min, the extraction temperature is70℃,the extraction time is90min.In the experiment, the complex enzyme’s mutual influence and the produce material theinfluence which responded to the enzymolysis need to carry on the deep step exploration.