Analysis Of Microbial Community Succession Of Denitrifying Phosphorus Removal Process

In recent years, people pay more and more attentions to the denitrifyingdephosphatation process, because of the reduction of organic carbon source andeconomy, and are ofgreat significance to reactor’s operation.For this situation, thisstudy sets the simulated domestic wastewater as process object. SBR was used todomesticate DPB, then achieves reactor stability andmake it the optimal condition toexanimate the domestication process and stabilize its phosphorus ability.PCR-DGGE technology was used to explore microbial community structure andsuccession, optimize microbial population, and establish the relationship betweenwork condition operating parameter and competition. Experimental results showthat：（1） PCR-DGGE technology can used on the bacterial population structure anddynamic change monitoring, combined with DGGE map, sequence homologyanalysis, map advantage strip cutting analysis showed that the developmental gluesequencing and system: the system operation process of microbial communitystructure changed significantly, the inoculation sludge microbial communities are themost abundant, but from the brightness of DGGE bands to determine biomass atleast, flora sludge inoculation and acclimation of PAO stage of the most similar, thebiggest difference with the colonies after enrichment with DPB stage, the dominantbacteria PAO were mainly Bacterium, Sphingobacteriaceae bacterium,Desulfurellaceae, and DPB stages of dominant bacteria are mainly Bacteroidetes andBacterium;（2） using NO₂-N as electron acceptor for Microbial Phosphorus RemovalSystem with domestication, sludge inoculation and stable phosphorus removalsludge homology is the worst, the diversity index increased slightly, the dominantbacteria species changed little but the number increased significantly, the dominantbands were sequenced by comparison, denitrification phosphorus removal dominantcolony for Bacteroidetes. （3） In different indicator system,phosphorus removal systems have the besteffect if DO indicator is controlled in2-3mg/L.It will be well running if the initialconcentration of NO₃-N and NO₂-N range in10mg/L-20mg/Lï¼›Though comparison,we find O₂is the best and NO₃-N the second in the different electron acceptordephosphorization system.The system with NO₃-N electron acceptor can fluctuatethe anaerobic release phosphorus and effluent. NO₂-N denitrifying dephosphatationis better than NO₃-N,though phosphorus removal effect is low;Respectively,the O₂,NO₃-N and NO₂-N phosphorus removal system structure succession analysis usingPCR-DGGE technology, the results showed that the system has advantages in sludgespecies common and their specific species, there are certain correlation between thetype and concentration of population structure and the electron acceptor in thesamples, using O₂as electron acceptor in phosphorus removal system structure isrelatively abundant, but the DGGE bands of brightness is not high, the total numberof colonies are not many, to NO₃-N for the total bacterial electron acceptor numberrichness of phosphorus removal system, and taking NO₂-N as the electron acceptorand phosphorus removal system of richness and diversity was lowest, whilephosphorus removal effect is the worst.