Study On Recognition Characteristics Of3,3’-Dichlorobenzidine Imprinted Monolithic Column

Monolithic column is a continuous column obtained by in situ polymerization preparation,also known as the continuous bed. People pay more and more attention to the monolithiccolumn technology in recent years. The monolithic column technology and the appearance ofthe further development of molecular imprinting technique, the template can be used in theproduction process of the imprinted monolithic column, in which the traditional grinding andother steps are omitted. The molecularly imprinted monolithic column is prepared based onmolecular imprinting coupled to monolithic column technology, expand the research field oftwo kinds of technology and increase the practicability of the original technology. Techniquesof molecular imprinted monolithic column has attracted many researchers, and is developingrapidly.Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) widespread inthe biosphere. The hydrogen atom on the benzene to biphenyl is replaced by chlorine atomsto form.3,3–dichlorobenzidine (DCB) which is an irreplaceable material of benzidineyellow and orange and. DCB is an animal carcinogen, can induce rat, mouse, hamster anddogs and other animal tumor, and it is the man suspected carcinogen.It is harm to theenvironment, resulting in water contamination. DCB as persistent organic pollutants, volatilemakes the pollution and harm has become the focus of attention. Therefore, the clear DCBenvironmental behavior and residual accurate detection in the environment of DCB is veryimportant to eliminate the harm.At present, DCB detection methods include ultraviolet spectrophotometric method, highperformance liquid chromatography. But these methods before the process is complex and thecost is high. Molecularly imprinted polymers (MIP) monolithic column has simplepreparation method, good selectivity and low cost, and has been widely used inchromatographic separation and solid phase extraction. For preparation of molecularlyimprinted monolithic columns, many organic solvents are used as porogen, thus strongrecognition of polar organic compounds are subject to certain restrictions. In this study, ionicliquid was used as the pore-forming agent and overcome the above shortcomings, andmolecularly imprinted monolithic column for the template DCB showed higher selectivity. The following studies are done in this paper.1. Using3,3-dichlorobenzidine as the template, methacrylic acid (MAA) as the functionalmonomer, azobisisobutyronitrile (AIBN) as a initiator, ethylene glycol dimethacrylate(EGDMA) as the cross-linker, BMIMPF6as the porogent, and the mixture of acetonitrile andchloroform as the solvent, a3,3-dichlorobenzidine imprinted monolithic column wasprepared by in situ molecular imprinting technique, and its binding properties and selectivitywere investigated.2. The binding character of DCB imprinted monolithic column was investigated and itexhibted specific recognition ability for DCB comparedted to its structural analogues underselected conditions．...