Development Of An Chemiluminescent Enzyme Immunoassay For Aflatoxin M₁

Aflatoxin M1exists widely in dairy products. With high toxicity and carcinogenicity, Aflatoxin M1has brought great harm to the health of people and has been listed as a carcinogen by the International Agency for Research on Cancer.This work takes the commercial AFM1antigen and antibody as raw materials, and uses horseradish peroxidase-Luminol-carbamide peroxide chemiluminescence system. A direct competitive method of CLEIA for AFM1has been initially built, and the conditions of the reaction system also optimized. A linear regression equation is y=24.417x+74.172. R2=0.9877. The sensitivity IC50of the method is0.01023ng/mL; the detection limit is0.00235ng/mL; the linear range is0.00604ng/mL-1.7326ng/mL.The established method has good precision and sample spike recovery. A simple CLEI A Kit has been assembled. A direct competitive ELISA method and an immunoaffinity column-HPLC method have been established by referring to GB and literature. It has been proved by experiments that CLEIA has good consistence with ELISA and HPLC in test results.The method in this work is rapid and accurate. It is of great significance in improving the detection technology of AFM1and controlling the occurrence of danger caused by AFM1.