The Establishment And Application Of High-throughput Biogenic Amines Detection System In Seafood

Bio genic amines are low molecular weight organic compounds containing nitrogen, more widely present in foods, especially protein-rich foods. In meat products, aquatic products and other protein-rich foods, the enzyme produced by a microorganism related to the catalytic removal of carboxyl groups and free amino acids to produce, and therefore is closely related to the content of biogenic amines and their quality is evaluated one such important food freshness indicators. The right amount of biogenic amines in the physiological activity of the body play an important regulatory role in, for example, regulation of body temperature, pH and volume control stomach, regulate human emotions, and polyamines in vivo protein, RNA and DNA synthesis plays an important role. But when the body after excessive intake of biogenic amines, will produce a series of emergency response, causing headaches, respiratory disorders, palpitations, changes in blood pressure and other adverse symptoms, the latest research shows that the biogenic amines in vivo cancer patients than in healthy people. Because of the potential toxicity of biogenic amines, excessive intake will have a greater human health hazards, and the biogenic amine content and quality of seafood have greater relevance. Current research on biogenic amines in foods has become a hot issue, its research and testing can enhance and improve food quality and safety.Currently detection of biogenic amines is mainly gas chromatography method capillary chromatography, liquid chromatography mass spectrometry and reversed-phase high-performance liquid chromatography. When using RP-HPLC method for the detection of biogenic amines, because neither of biogenic amines in itself is no specific fluorescence characteristics of UV absorbing group, generally require pre-column or post-column derivatization, the process is relatively cumbersome. Anion exchange ion chromatography separation principle biogenic amines, pulse integration ampere (PDA) electrochemical detection of biogenic amines for direct detection method is simple, and highly sensitive, more suitable for application.This paper has established the alkaline pulse after ion exchange chromatography column integral ampere method determination of dopamine in frozen seafood, tyramine, serotonin, putrescine, cadaverine, histamine, guanidine butylamine, phenethylamine, and spermine, spermine and other10kinds of biogenic amine method. Samples after acid solution to extract the centrifugal in IonPacCS18analysis column and IonPacCG18protect column separation, the gradient changes of methyl sulfonic acid (MSA) as eluent, flow rate of1.3ml/min, the column temperature40℃, column after join0.1mol/L NaOH as derivative reagent, alkali solution pulse integral ampere detector. The results showed that the baseline separation of ten kinds of biogenic amines can achieve in a certain concentration range, good linearity, detection limit, within50ug/kg rate between86.0%and108.0%, the RSD of retention time and peak area of sample is less than1%and5%RSD (n=7). This method is suitable for the determination of different kinds of biogenic amine separation in frozen seafood.