The layer structure of Graphenequalities it for large specific surface area and excellent adsorption potential. Graphite Oxide, which is a precursor of graphene, not only contains large surface area, but also have multiple oxygen-containing functional groups and excellent adsorption potential for aromatic compounds. This thesis studied adsorption of tetracycline in honey matrix that can be base application for the detection and removal of residual tetracycline in honey and relative products,Improved Hummers oxidation method was used to prepare Graphite Oxide with good dispersion state and adsorption capacity. Research on the effect of ultrasonic time display that240min ultrasound processing could achieve better separation result. To prepare a Graphite Oxide filled solid phase extraction column, microcrystalline cellulose was selected as the filter. This analyical column could reach an average adsorption efficiency of98.96%, column efficiency of1.979μg for2μg tetracycline(n=54).The adsorption isotherm of tetracycline and effects of factors to the adsorption of tetracycline with GrapheneOxide in water matrix study showed that Langmuir equation fit best for the adsorption process, adsorption on Graphite Oxide reduced with the rise of pH. These indecated that the main way og adsorption for Graphite Oxide in low concentration tetracycline system is monolayer adsorption, the major contribution is electrostatic interactions between the Graphite Oxide surface and tetracycline molecules, followed may also exist π-π interaction.Dispersed adsorption and micro pocket adsorptionof tetracycline with Graphite Oxide showed that:the presence of litchi honey just reduced Graphite Oxide adsorption rate of tetracycline and extended the adsorption equilibrium time, but did not affect the absorption efficiency of tetracycline; the tight adsorption of tetracycline in low concentrations honey matrix could be achieved by micro pocket. Further research of the material of Micro poket is needed to realise the removal application of residual tetracycline in honey. |