Study On The Protective Effect Of Procyanidins On The Human Trabecular Meshwork Cells

Objective：To investigate the protection and mechanism of Procyanidins (PC) againsthydrogen peroxide induced oxidative damage of Human Trabecular MeshworkCells(HTMC) in order to provide an experimental foundation for glaucoma clinicaltreatment.Methods：Human Trabecular Meshwork Cells were cultured and then divided randomlyinto5groups. As untreated group: normal cultured HTMC; the control group: thenormal cultured HTMC+hydrogen peroxide; the treated group: the normal culturedHTMC+hydrogen peroxide+PC (PC final concentrations were0.02mg/ml,0.05mg/ml,0.10mg/ml). To investigate the expression of mitochondrial complexI mRNAwith real-time fluorescence quantitative polymerase chain reaction (PCR), detectingthe cell apoptosis rates by flow cytometry and using the Western blot test detected thereleasing of cytochrome C (CytC).Results：1. The differences between PC treated groups and control group werestatistically significant (P <0.01), which is showed HTMC treated with PC canincrease the expression of mitochondrial complex Ⅰ mRNA, reduce the releasing ofCytC and the cell apoptosis rates.2. The differences in each PC treated groups werestatistically significant (P <0.01), which is showed the expression of mitochondrialcomplex Ⅰ mRNA were increasing, the releasing of CytC and the cell apoptosis rateswere reducing along with the concentration of PC gradually increasing.3. Thedifferences of the cell apoptosis rates between PC treated groups and untreated groupwere statistically significant (P <0.01), Compared with untreated group, thedifferences of the expression of mitochondrial complex Ⅰ mRNA and releasing ofCytC in0.02mg/mlPC,0.05mg/mlPC groups were statistically significant (P <0.01),but the0.10mg/mlPC group had no significant differences (P>0.05). Conclusion：Exogenetic PC has a strong antioxidant effect which can increase the expressionof the oxidative damage human trabecular meshwork cells mitochondrial complex ⅠmRNA, reduce the releasing of CytC and the cell apoptosis rates, and in a certainrange of concentration, the protective effect of PC have the positive relationship ofdose-effect, These findings suggest that PC may be a good candidate for further studyof the clinical treatment of glaucoma.