The Association Study Of MYH9Gene Single Nucleotide Polymorphism And Chronic Kidney Disease In Inner Mongolia

Objective Analyze and compare the clinical features of chronic kidney diseasedeferent stages,to Study on the Relationship between the single nucleotidepolymorphism (SNP) in MYH9and clinical characteristics and progression in patientswith chronic kidney disease of Han nationality in Inner Mongolia autonomous region.Method Collect301patients who were proved to CKD and294health controls, Theperipheral blood of patients from the above extract DNA.The SNP in MYH9gene ofRs3752462、 Rs4821480site were determined by PCR-restriction fragment lengthpolymorphisms (PCR-RFLP). Compare the differences of the sites in chronic kidneydisease and the control group; Analysis the association of different genotypes and clinicalfeatures of CKD, the association of Different genotypes with CKD Different primarydisease,;Analysis of the relationship between different genotypes associated with diseaseprogression of chronic kidney disease Rs3752462sites..Statistical analysis.werepreformed by SPSS17.0version.Result (1) Rs3752462site groups、 RS4821480site groups in Hardy Weinbergequilibrium (P>0.05)(2) CKD2period and CKD4、5diastolic pressure was statisticallysignificant difference(P <0.05), CKD2period significantly lower than CKD4,5;CKD3period and CKD4,5platelet was significant difference (P <0.05) CKD3plateletsignificantly higher than CKD4,5phase; Age, systolic blood pressure,24hour urinaryprotein, blood, serum uric acid quantitative high density lipoprotein has no significantdifference (P>0.05)(3) In the CKD group serum total cholesterol(TC), low-densitylipoprotein (LDL)-L), triglycerides (TG) between groups was statistically significantdifference compared (P <0.05) with CKD progress TC, LDL-L, TG drop(4) systolicblood pressure (SBP) level of Rs3752462site CT genotype of patients significantly higher than CC genotype of patients (P <0.05)(P=0.036); glomerular filtration rate(eGFR) level of CC genotype of patients significantly higher than the genotype CTpatients (P <0.05)(P=0.045), CC genotype TT is significantly higher than the patientsgenotype of patients (P <0.05)(P=0.048)(5) two sites CKD group and control groupgenotype and allele frequency distribution is no significant differences(6) Rs3752462siteCKD1-5each group compared with controls for genotype and allele frequencydistribution is no significant differences(7) glomerular filtration rate and blood serumcreatinine, albumin, age was negatively correlated,with hemoglobin, blood cholesterol,triglyceride into positive correlation.(8) Rs3752462site CC genotype is caused increasedprotection factor CKD systolic blood pressure; CC gene type is cause illness developmentof ESRD CKD to independent protection factor, gene mutations in C for the occurrenceof ESRD T may cause.Conclusion (1).There are the association between MYH9gene polymorphism andchronic kidney disease in Inter Mongolia,adding to the mounting evidence of MYH9asan candidate gene in CKD pathogenesis(2)The CKD patients who carry MYH9genesRs3752462site CC genotype are not susceptible to high blood pressure, CC gene type iscause for patients with CKD systolic blood pressure increased protection factor, genemutations in C for T can lead to systolic blood pressure increased.(3)MYH9geneRs3752462site CC genotype is an independent protection factor for ESRD, genemutations in C allele to T allele may cause. ESRD(4).Due to toxins, patients withend-stage renal disease lead to platelet population decline(5) CKD patients have lipidmetabolism disorders performance such as the triglycerides (TG) increase, high-densitylipoprotein (HDL-C) reduce, but with the disease developing, TG is increasing ordiscreasing need be further proved.