The Research On The Relationship Between FKBP5Gene Polymorphisms In Locus Rs1360780and The Response To Steroid Therapy In Children With Nephrotic Syndrome

Object:The aim of the study was to investigate the relationship between FKBP5gene polymorphisms in locus rs1360780and the response to steroid therapy in children with primary nephrotic syndrome.To further explore the mechanism of steroid-resistant,and expect the results can detect the genotype associated with steroid-resistant.To predict the response to steroid therapy and disease progression in children with primary nephrotic syndrome.Method:Fifty children with primary nephrotic syndrome (PNS) were enrolled as case group,100healthy children were added as control group. According to the clinical manifestations, the case group were divided into simple NS group(29case)and nephritic NS group(21case). According to the response to steroid therapy,the case group were divided into steroid resistance NS group(17cases)and non-steroid resistance NS group(33cases). All subject were genotyped for FKBP5polymorphisms in locus rs1360780by using polymerase chain reaction (PCR) and direct gene sequence test technique. At the same time monitoring24hours urine protein quantity, plasma albumin, blood urea nitrogen, serum creatinine, plasma total cholesterol, triglyceride, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol.Results:1The frequencies of TT,TC,CC genotype on case group and control group were16.0%,40.0%,44.0%and7.0%,41.0%,52.0%respectively;and the frequency of T and C allele on case group and control group were36.0%,64.0%and27.5%,72.5%respectively. There were no significant differences in genotype and allele frequence distribution between the two groups(χ2=3.14,P=0.21;χ2=2.28,P=0.14).2The frequencies of TT,TC,CC genotype on simply NS group and nephritic NS group were10.3%,38.0%,51.7%and23.8%,42.9%,33.3%respectively,and the frequency of T and C allele on simply NS group and nephritic NS group were29.3%,70.7%and45.2%,54.8%respectively. There were no significant differences in genotype and allele frequence distribution between the two groups(χ2=2.34,P=0.35;χ2=2.08,P=0.14).3The frequencies of TT,TC,CC genotype in steroid resistance NS group and non-steroid resistance NS group were5.9%,58.8%,35.3%and21.2%,30.3%,48.5%respectively,and the frequency of T and C allele on steroid resistance NS group and non-steroid resistance NS group were35.3%,64.7%and36.4%,63.6%respectively.There were no significant differences in genotype and allele frequence distribution between steroid resistance NS group and non-steroid resistance NS group,steroid resistance NS group and control group,non-steroid resistance NS group and control group(χ2=4.04,P=0.14;χ2=1.92,P=0.38;χ2=5.09,P=0.08and χ2=0.01,P=0.92;χ2=0.86,P=0.41;χ2=1.87,P=0.21).4Comparisons of24hours urine protein, plasma albumin, blood urea nitrogen, serum creatinine,plasma total cholesterol, triglyceride, high-density lipoprotein cholesterol and low density lipoprotein cholesterol of different FKBP5genotypes in patients were not significantly different(F=0.15,P=0.86;F=0.56,P=0.58;F=0.67,P=0.52;F=0.28,P=0.76;F=0.12,P=0.89;F0.10, P=0.90;F=1.75,P=0.19;F=0.22,P=0.81).Conclusion:1There were no relationship between FKBP5gene polymorphisms in locus rs1360780and steroid resistance in children with primary nephrotic syndrome.2There were no relationship between FKBP5gene polymorphisms in locus rs1360780and the morbidity,clinical manifestations in children with primary nephrotic syndrome.3In case group,there were no relationship between three different genotypes of FKBP5gene in locus rs1360780with24hours urine protein quantity, plasma albumin, blood urea nitrogen, serum creatinine,plasma total cholesterol, triglyceride, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol.