Study Of Relationship Between Dopamine D1Receptor Gene-48A/G Polymorphism And Cognitive Dysfunction After Frontal Lobe Contusion

Objective TO investigate the relationship between dopamine D1receptor gene-48A/G polymorphism and cognitive dysfunction after frontallobe contusion.Methods About120Healthy controls and104patients with frontal lobecontusion after3to6months conservative treatment as the case group areevaluated the cognitive function by using Loewenstein OccupationalTherapy Cognitive Assessment （LOCTA） and Wiscosin Cards SortingTest(WCST).Extract the genomic DNA of the104case group and120healthy control group, and detect the genotype of DRD1gene (-48A/G)polymorphism by the polymerase chain reaction restriction fragment lengthpolymorphism analysis (PCR-RFLP). Then using Statistic Package forSocial Science(spss) to study the relationship between DRD1gene (-48A/G)polymorphism and the cognitive dysfunction of patients with mild/moderatefrontal lobe contusion.Results By the statistical tests, the differences between the case groupand control group has no statistical significance (P>0.05)in sex, age, level ofeducation, right-handed. Two groups on the genotype distribution and allelefrequency has no significant difference (P>0.05); the genotype distributionconforms to the laws of Hardy Weinberg equilibrium (P>0.05), and it isrepresentative. Compared with the normal control group in LOTCA, patientswith left frontal lobe damage has decreased significantly (P<0.05)in spatialperception, visual movement tissue, logic thinking; in addition to spatialperception, visual movement tissue, logical thinking, attention focus is also significantly decreased (P<0.05) in patients with right frontal lobe injury;and patients with bilateral frontal lobe contusion are decreased significantly(P<0.05) in orientation, visual perception, spatial perception, visualmovement tissue, logical thinking and attention focus function. But inWCST, three group patients (left, right, bilateral group) have significantdifference (P<0.05)with the controls， include persistent responses,continuity errors, percent conceptual level, number of categoriescompleted,and the required responses on completion of the firstclassification. Compared with the control group in LOTCA, type AA weresignificantly different (P<0.05) in the orientation， spatial perception, visualmovement tissue, logical thinking, attention focus.And type AG hadsignificant difference in spatial perception, visual movement tissue,andlogical thinking, and there was no statistically significant difference (P>0.05)between GG type and the control group. when compared with thehealthy control group of WCST, only GG genotype have no significantdifference (P>0.05)in the number of persistent errors, conceptual level, thepercentage of completed categories, there were significant differences (P<0.05) in the remaining type items. As a risk factor for the A, OR=1.041，95%CI=0.620~1.747.Conclusions Wiscosin Cards Sorting Test(WCST) is sensitive forfrontal lobe brain damage, it can reflect the subject’s cognitive functionaccurately, especially in the concept of the formation and metastasis. Thedamage parts of the frontal lobe is significant associated with theperformance of cognitive dysfunction. There is a certain correlationbetween DRD1(-48A/G) polymorphism and cognitive impairment. Allele Amay be one of the risk factors as patients with cognitive dysfunction.