Effect Of Osteoblast Adhesion On Ti6Al7Nb Materials With Different Morphologies

Titanium and titanium alloys as biomaterials have been widely used in themedical field, especially in orthopaedics, oral surgery and cardiovascular surgery.As an ideal implant material, Ti6Al7Nb must have meet the excellentbiocompatibility to form good interface with body internal environment. Thesurface topography is the main factor influencing the interaction betweenbiomaterial surface and organism. The compatibility of the biomaterials in thebody can be improved by making implant surface structure patterned design, thusto promote bone integration function. Therefore, it is significant to make acomprehensive evaluation of Ti-material surface topography and study celladhesion, proliferation and cell morphology.In this article, different processing methods were developed to producepolishing, grinding, and sand-blasting surface topography to compare theinfluence of the surface topography on osteoblasts (MC3T3) adhesion,proliferation and morphology on Ti6Al7Nb substrates. The experimental resultsshowed that surface topography of titanium impacted on osteoblast adhesion,spreading and proliferation, then ultimately obtained the influence regularity ondifferent surface roughness and fractal dimension to biological behaviors.MTT and cell couting method was used to assay the osteoblasts adhesionand proliferation. The surfaces with rougness of Ra=2.323±0.056μm,Rz=16.915±0.543μm were showed a good affinity for MC3T3osteoblasts in theearly stage from the surface topography amplitude angle. The smoother androugher surfaces were observed a low rate of cell adhesion. The smoothestsurfaces were conducive to the proliferation of osteoblasts. We utilized fractaltheories to calculate the titanium surface fractal dimension.The greater fractaldimension is, the more irregular the surface shows. The cell culture experimentalanalysis indicated that cell adhesion and proliferation rate decreased when thesurface fractal dimension increased. Cell morphology was not the same on thedifferent surfaces and the polished surface was propitious to spread. With theincubation time increasing cells displayed long spindle, more filopodiums andthe cell layers were confluent and appeared paving way when cell morphology was irregular polygons at the early stage. It was obvious that cells oriented in theparalled way on polished and mechanical machine surfaces, calling contact guide.On sandblasted surfaces with their sharp ridges cell morphology were influencedand their multi-fingered lamellipodias attached the raised edge or converged withothers across nearby trenches.