| Objective Mechanism of plasma cell mastitis (PCM) is still not clear up to now. Thepathological diagnosis is gold standard.This study using the thymosin a1interventionserological immune markers plasma cell mastitis rabbit model before and after the change(including immunoglobulin IgG, IgA, IgM, CD4/CD8positive cells percentage) to explorethymosin a1pulpcell mastitis immune mechanisms. This study through the use of patientplasma cell mastitis sterile diseased tissue homogenates inoculated into rabbits to inducelesions and detection of lesions in rabbits serological immune indicators change, analysis ofplasma cell mastitis occurrence of the development of B, T lymphocytes and macrophageschanges possible relationship with the disease further to investigate plasma cell mastitisimmune pathogenesis, and thymosin a1provide a theoretical basis for the treatment of plasmacell mastitis.Methods (1) Material Source: collect the the Ningxia region cut lesions in patientswith plasma cell mastitis surgery; standard rabbit peers, with similar weight nonpregnantfemale rabbits (purchased from the Experimental Animal Center of Ningxia Medical)35.Randomly divided into4groups, Saline group, normal breast tissue homogenates, modelgroup, not injecting drug model group, model group were given the drugs were injectedsubcutaneously with normal breast tissue homogenates group5, the remaining10in eachgroup (2) by parts to the mammary glands of rabbits injected with plasma cell mastitis sterileof necrotic tissue homogenates induced by plasma cell mastitis animal models. Pathologicaltesting to confirm the lesion formation.(3) Thymosin a1intervention: Thymosin a1subcutaneous injection into lesions in rabbits. Before treatment, after different periods takenvenous serum of rabbits.By using ELISE kit and different periods of flow cytometry to detect serum of rabbits immune markers change.(4) Statistics: SPSS18.0statistical package, inwhich the rate of inspection using the chi-square test, α=0.05level of inspection.Result The pathological results show:7d-14d model rabbits ductal significantexpansion within the deposition of a large number of epithelial cells and necrotic debrisperitubular fibrosis, and a large number of neutrophils, lymphocytes, especially plasma cellinfiltration.Immunological detection results: planting7d,14d when detecting normal groupand do not inject drugs model group, model group rabbit serum IgA, IgG, IgM,CD4~+/CD8~+of content, plant7d serum IgA, IgG, IgM,CD4~+/CD8~+change was statisticallysignificant (P <0.05); the the14D test results IgA, IgG, CD4~+/CD8~+content was notstatistically significant (P <0.01), IgM content was statistically significant (P <0.05).Thymosin a1injection group and non-injection drug group immune parameters statisticalresults contrast injection therapy drug thymosin a13d after IgA, IgG, IgM content changeswere not statistically significant; CD4~+/CD8~+was statistically significant (P <0.05). Injectionfor7days after the CD4~+/CD8~+There are statistically significant, IgA, IgG, IgM was notstatistically significant.Conclusion lesions of patients with plasma cell mastitis induced female the rabbit breast tissue appears similar pathological clinical performance. Model rabbit serum IgA, IgG, IgM, CD4~+/CD8~+content in different periods of significant differences. |
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